Source:http://linkedlifedata.com/resource/pubmed/id/12480114
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2002-12-13
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pubmed:abstractText |
The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C(4)-C(8)) substrates, and poor activity on C(18) substrates. On the basis of amino acid sequence homology, the cloned lipase was classified into subfamily I.4 of bacterial lipases.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0378-1097
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2002 Federation of European Microbiological Societies
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
217
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
263-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12480114-Bacillus megaterium,
pubmed-meshheading:12480114-Bacterial Proteins,
pubmed-meshheading:12480114-Base Sequence,
pubmed-meshheading:12480114-Cloning, Molecular,
pubmed-meshheading:12480114-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12480114-Genes, Bacterial,
pubmed-meshheading:12480114-Hot Temperature,
pubmed-meshheading:12480114-Hydrogen-Ion Concentration,
pubmed-meshheading:12480114-Lipase,
pubmed-meshheading:12480114-Lipolysis
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pubmed:year |
2002
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pubmed:articleTitle |
Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase.
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pubmed:affiliation |
Department of Microbiology, Faculty of Biology, University of Barcelona, Av Diagonal 645, 08028 Barcelona, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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