Linked Life Data

12467924

Source:http://linkedlifedata.com/resource/pubmed/id/12467924

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-12-6
pubmed:abstractText
Reactions of primary amines with salicylaldehyde-5-sulfonate (SAS) lead to the formation of stable Schiff bases at weakly alkaline pH and at moderate temperature in an ethanol-rich aqueous solution. Some alkane diamines were converted to salicylaldimine with SAS, and the products were resolved and detected by capillary zone electrophoresis (CZE). The reactivity of SAS was applied also to the derivatization of amino acids. Optimal conditions for the analysis of lysine in the presence of diaminopimelic acid, the metabolite of lysine, were investigated. Addition of Na(2)SO(4) into the migrating solution enhanced the stacking effect, and led to increase in the detection limits. Another advantage of the use of SAS is its ability to form stable water-soluble metal chelate as Schiff base ligands. For practical analysis, the copper chelate of the Schiff base of isoniazid, which is known as the most active antibacterial used in the treatment of pulmonary tuberculoses, was investigated, and the detection limit of isoniazid was improved in the magnitude of 11 times compared with the one of free isoniazid.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1523-30
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Investigation of salicylaldehyde-5-sulfonate as a precolumn derivatizing agent for the determination of n-alkane diamines, lysine, diaminopimelic acid, and isoniazid by capillary zone electrophoresis.
pubmed:affiliation
Department of Chemistry, Faculty of Science, Okayama University, 3-1-1 Tsushimanaka, Okayama, Japan.
pubmed:publicationType
Journal Article