Linked Life Data

12465949

Source:http://linkedlifedata.com/resource/pubmed/id/12465949

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
49
pubmed:dateCreated
2002-12-5
pubmed:abstractText
Several chlorinated organic compounds (COCs) that have been detected in a wide range of human, animal, and environmental samples may be derived from natural or anthropogenic sources. To determine whether the Cl isotope ratios of these compounds could be used to differentiate sources, we investigated the chlorine isotope effect for enzyme-catalyzed chlorination. Two aromatic substrates, 1,3,5-trimethylbenzene (TMB) and 3,5-dimethylphenol (DMP), were treated with a chloroperoxidase isolated from the fungus Caldariomyces fumago. A kinetic isotope effect (KIE) (in terms of k35/k37) was calculated to be 1.012 for TMB and 1.011 for DMP. A similar reaction, but not catalyzed, with hypochlorite yielded a much smaller KIE. These results indicate that a substantial KIE exists for this process. Furthermore, natural COCs synthesized by this enzymatic pathway may have Cl isotope ratios that will be easily distinguished from anthropogenic COCs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0002-7863
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
14526-7
pubmed:dateRevised
2008-1-17
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
A chlorine isotope effect for enzyme-catalyzed chlorination.
pubmed:affiliation
Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, USA. creddy@whoi.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't