Source:http://linkedlifedata.com/resource/pubmed/id/12461139
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
2002-12-3
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pubmed:abstractText |
Polygalacturonate 4-alpha-galacturonosyltransferase (GalA T) activity was detected in the microsomal fraction isolated from pumpkin (Cucurbia moschata Duchesne, cv. Tokyou-Kabocha) seedlings using UDP-GalA and 2-aminobenzamide (2AB)-labeled oligogalacturonides. A 2AB-labeled undecagalacturonide was elongated by the attachment of galacturonic acid (GalA) residues to give 2AB-labeled oligogalacturonides with a degree of polymerization (DP) between 12 and 17. Exogenous 2AB-labeled oligogalacturonide acceptors with a DP >3 are effective acceptor molecules for pumpkin GalA T.
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pubmed:language |
eng
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anthranilic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Glucuronosyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/UDP-galacturonic acid,
http://linkedlifedata.com/resource/pubmed/chemical/Uridine Diphosphate Sugars,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-1,4-galacturonosyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/anthranilamide,
http://linkedlifedata.com/resource/pubmed/chemical/oligogalacturonic acid
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pubmed:status |
MEDLINE
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pubmed:author | |
pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1386-9
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pubmed:dateRevised |
2006-11-15
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pubmed:articleTitle |
A sensitive and rapid bioassay of homogalacturonan synthase using 2-aminobenzamide-labeled oligogalacturonides.
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pubmed:affiliation |
Forestry and Forest Products Research Institute, P. O. Box 16, Tsukuba Norin Kenkyu Danchi-nai, Ibaraki, 305-8687 Japan. tishii@ffpri.affrc.go.jp
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