12451714

Source:http://linkedlifedata.com/resource/pubmed/id/12451714

Download in:

Switch to

Custom View

Named Graph Language Inference

Statements in which the resource exists as a subject.
Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0012634, umls-concept:C0015733, umls-concept:C0029235, umls-concept:C0032520, umls-concept:C0314919, umls-concept:C1511790
pubmed:dateCreated	2002-11-27
pubmed:abstractText	We examined whether the Tyzzer's disease organism, Clostridium piliforme, could be detected in feces by PCR. If the organism could be detected in feces, a diagnosis could be made without sacrifice of the animal. Using the RT strain of C. piliforme, we found that a C. piliforme band could be detected when there were > or = 1 x 10(0) bacteria present in the PCR solution, but the presence of fecal extract in the solution depressed the sensitivity 10 fold. Nevertheless, we could detect the C. piliforme-specific band in fecal extracts from rats in a naturally infected colony, and concluded that the use of PCR to detect C. piliforme DNA in fecal extracts would be a useful diagnostic technique.
pubmed:language	eng
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial
pubmed:status	MEDLINE
pubmed:author	pubmed-author:FujiedaMitsuhiroM, pubmed-author:FurukawaToshinoriT, pubmed-author:FurumotoKayoK, pubmed-author:OkadaEikoE
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	513-6
pubmed:dateRevised	2003-11-14
pubmed:articleTitle	Detection by PCR of the Tyzzer's disease organism (Clostridium piliforme) in feces.
pubmed:affiliation	Research Facilities for Laboratory Animal Science, Hiroshima University, School of Medicine, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan.