Source:http://linkedlifedata.com/resource/pubmed/id/12421569
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2002-11-7
|
| pubmed:abstractText |
Synergism between stem cell factor (SCF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) has been shown to be essential for hematopoietic cell proliferation. Since HML-2 cells proliferate exponentially in the presence of SCF and GM-CSF together, we analyzed the molecular mechanism of the interaction between these two factors in the cells. An immediate-early gene product, c-myc, was additively upregulated in HML-2 cells by addition of a combination of SCF and GM-CSF. c-myc antisense oligonucleotides effectively suppressed cell proliferation and downregulated the induction of D3, E, A, and B cyclins in HML-2 cells stimulated with the two-factor combination. HML-2 cells arrested at the G0/G1 phase with SCF alone and expressed modest amounts of c-myc and cyclin D3, but not cyclin E. With GM-CSF treatment alone, the cells could not progress to the G2/M phase and expressed c-myc, cyclin D3 and cyclin E but not cyclins A or B. The addition of the counterpart cytokine resulted in cell cycle completion by induction of the deficient cyclins. Taken together, it appears that the induction of c-myc is an indispensable event in the proliferation of HML-2 cells and that the cytokines SCF and GM-CSF interact reciprocally for expression of all cyclins required for cell cycle progression.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
1043-4666
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
21
|
| pubmed:volume |
19
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
267-75
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12421569-Cell Cycle,
pubmed-meshheading:12421569-Cell Division,
pubmed-meshheading:12421569-Cyclins,
pubmed-meshheading:12421569-Drug Synergism,
pubmed-meshheading:12421569-G1 Phase,
pubmed-meshheading:12421569-G2 Phase,
pubmed-meshheading:12421569-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:12421569-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:12421569-Hematopoietic Stem Cells,
pubmed-meshheading:12421569-Humans,
pubmed-meshheading:12421569-Immediate-Early Proteins,
pubmed-meshheading:12421569-Leukemia, Megakaryoblastic, Acute,
pubmed-meshheading:12421569-Mitosis,
pubmed-meshheading:12421569-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:12421569-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:12421569-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:12421569-S Phase,
pubmed-meshheading:12421569-Stem Cell Factor,
pubmed-meshheading:12421569-Tumor Cells, Cultured
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Synergism between stem cell factor and granulocyte-macrophage colony-stimulating factor on cell proliferation by induction of cyclins.
|
| pubmed:affiliation |
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan. tkamijo@hsp.md.shinshu-u.ac.jp
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|