Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2002-11-5
pubmed:abstractText
Several aspects of eukaryotic mRNA processing are linked to transcription. In Saccharomyces cerevisiae, overexpression of the mRNA export factor Sub2p suppresses the growth defect of hpr1 null cells, yet the protein Hpr1p and the associated THO protein complex are implicated in transcriptional elongation. Indeed, we find that a pool of heat shock HSP104 transcripts are 3'-end truncated in THO complex mutant as well as sub2 mutant backgrounds. Surprisingly, however, this defect can be suppressed by deletion of the 3'-5' exonuclease Rrp6p. This indicates that incomplete RNAs result from nuclear degradation rather than from a failure to efficiently elongate transcription. RNAs that are not degraded are retained at the transcription site in a Rrp6p-dependent manner. Interestingly, the addition of a RRP6 deletion to sub2 or to THO complex mutants shows a strong synthetic growth phenotype, suggesting that the failure to retain and/or degrade defective mRNAs is deleterious. mRNAs produced in the 3'-end processing mutants rna14-3 and rna15-2, as well as an RNA harboring a 3' end generated by a self-cleaving hammerhead ribozyme, are also retained in Rrp6p-dependent transcription site foci. Taken together, our results show that several classes of defective RNPs are subject to a quality control step that impedes release from transcription site foci and suggest that suboptimal messenger ribonucleoprotein assembly leads to RNA degradation by Rrp6p.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-10207065, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-10323864, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-10359759, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-10611239, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-10668806, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-10722314, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-10786854, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-10952996, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11014198, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11030346, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11030620, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11060033, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11118221, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11142374, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11156602, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11156603, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11156604, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11158589, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11336711, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11459827, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11463828, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11532962, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11564888, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11586364, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11675789, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11675790, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11696331, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11696332, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11756420, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11805837, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11849973, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11909521, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11932736, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-11979277, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-12022234, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-7744966, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-9242493, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-9390555, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-9407037, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-9774696, http://linkedlifedata.com/resource/pubmed/commentcorrection/12417728-9891041
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphatases, http://linkedlifedata.com/resource/pubmed/chemical/Exoribonucleases, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/HPR1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Heat-Shock Proteins, http://linkedlifedata.com/resource/pubmed/chemical/HsP104 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/RNA-dependent ATPase, http://linkedlifedata.com/resource/pubmed/chemical/RRP6 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8254-66
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Interactions between mRNA export commitment, 3'-end quality control, and nuclear degradation.
pubmed:affiliation
Centre National de la Recherche Scientifique, Centre de Genetique Moleculaire, 91190 Gif-sur-Yvette, France. Libri@cgm.cnrs-gif.fr
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.
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