Linked Life Data

12417668

Source:http://linkedlifedata.com/resource/pubmed/id/12417668

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2002-11-5
pubmed:abstractText
Spontaneous neuronal activity is essential to neural development. Until recently, neurons were believed to be the only excitable cells to display spontaneous activity. However, cultured astrocytes and, more recently, astrocytes in situ are now known to exhibit spontaneous Ca2+ transients. Here we used Ca2+ imaging of astrocytes from transgenic mice for the simultaneous monitoring of [Ca2+]i changes in large numbers of astrocytes. We found that spontaneous activity is a common property of most brain astrocytes that is lost in response to a lesion. These spontaneous [Ca2+]i oscillations require extracellular and intracellular Ca2+. Moreover, network analysis revealed that most astrocytes formed correlated networks of dozens of these cells, which were synchronous with both astrocytes and neurons. We found that decreasing spontaneous [Ca2+]i transients in neurons by TTX does not alter the number of active astrocytes, although it impairs their synchronous network activity. Conversely, bicuculline-induced epileptic patterns of [Ca2+]i transients in neurons cause an increase in the number of active astrocytes and in their network synchrony. Furthermore, activation of non-NMDA and NMDA ionotropic glutamate receptors is required to correlate astrocytic networks. These results show that spontaneous activity in astrocytes and neurons is patterned into correlated neuronal/astrocytic networks in which neuronal activity regulates the network properties of astrocytes. This network activity may be essential for neural development and synaptic plasticity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9430-44
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12417668-Animals, pubmed-meshheading:12417668-Astrocytes, pubmed-meshheading:12417668-Biological Clocks, pubmed-meshheading:12417668-Brain, pubmed-meshheading:12417668-Calcium, pubmed-meshheading:12417668-Calcium Channel Blockers, pubmed-meshheading:12417668-Calcium Signaling, pubmed-meshheading:12417668-Chelating Agents, pubmed-meshheading:12417668-Extracellular Space, pubmed-meshheading:12417668-Glial Fibrillary Acidic Protein, pubmed-meshheading:12417668-Green Fluorescent Proteins, pubmed-meshheading:12417668-Hippocampus, pubmed-meshheading:12417668-Intracellular Fluid, pubmed-meshheading:12417668-Luminescent Proteins, pubmed-meshheading:12417668-Mice, pubmed-meshheading:12417668-Mice, Transgenic, pubmed-meshheading:12417668-Models, Neurological, pubmed-meshheading:12417668-Monte Carlo Method, pubmed-meshheading:12417668-Nerve Net, pubmed-meshheading:12417668-Neurons, pubmed-meshheading:12417668-Receptors, Glutamate, pubmed-meshheading:12417668-Recombinant Fusion Proteins, pubmed-meshheading:12417668-Synaptic Transmission, pubmed-meshheading:12417668-Tetrodotoxin
pubmed:year
2002
pubmed:articleTitle
Neuronal activity regulates correlated network properties of spontaneous calcium transients in astrocytes in situ.
pubmed:affiliation
Department of Cell Biology and Barcelona Science Park, University of Barcelona, Barcelona E-08028, Spain. aguado@medicina.ub.es
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't