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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-11-4
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pubmed:abstractText |
Proliferation and differentiation of B cells has been extensively studied and the study of feedback suppression of B cell proliferation has been limited to humoral factors. However, very little is known about feedback suppression of B cell proliferation by cellular influences. We have previously reported on the role of T cells and their subsets on B cell proliferation in that we did not observe suppression of B cell colony growth by T cells. We now report on the role of B cells in limiting B cell proliferation. B cell colonies were grown in methyl cellulose for either 3 days or 5-6 days utilizing 2 x 10(5) T cells irradiated with 9,000 rads, and 2 x 10(5) B cells. The B cells were then obtained from these colonies and increasing numbers of cells were added to fresh autologous B cells that were further cultured for 5 days to form new B cell colonies. At the end of this period, B cell colony numbers were determined. Our data show that addition of CD19- and CD20- positive B cells recovered from mature colonies after 5 days to fresh B cells suppressed further B cell colony growth in all cases tested, whereas addition of CD19-positive B cells recovered from immature colonies after 3 days of culture did not suppress further B cell colony growth. Elimination of CD 19- or CD20-positive cells with monoclonal antibody to CD19 and complement or by the technique of panning enhanced colony growth. Supernatants obtained from B cell colonies did not suppress B cell colony formation. Our data suggest that there is feedback suppression of normal progenitor B cell proliferation by constituent B cells and that this effect develops during maturation of colonies during the growth phase.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-1086776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-2415587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-2437199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-2935205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-3018960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-3079612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-3088118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-308929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-3258550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-3485142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-3494785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-3500237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-3925015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-3933529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6092473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6164922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6172514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6177765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6217247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6224523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6276467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6415207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6457863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6459291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6609082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6976808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12412767-6979564
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
314-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1989
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pubmed:articleTitle |
Feedback suppression of B cell colony formation in healthy individuals.
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pubmed:affiliation |
Department of Medicine, Camp Hill Hospital, Dalhousie University, Halifax, Nova Scotia, Canada.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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