Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2002-11-4
pubmed:abstractText
Lipoxin A4 (LXA4) has been shown to bind to the leucocyte formyl peptide receptor (FPR) homologue, FPRL1, without triggering the biological activities induced by other FPRL1 agonists. We investigated the direct effect of LXA4 as well as the effect on agonist-induced biological responses using transfected HL-60 cells expressing FPR, FPRL1 or FPRL2. LXA4 neither induced an intracellular rise in calcium in these transfectants nor affected the response induced by the peptide Trp-Lys-Tyr-Met-Val-Met (WKYMVM), an agonist that activates cells through FPRL1 and -2. Both agonists induced Erk-2 activation; however, the eicosanoid-induced activity was independent of FPRL1 and FPRL2. Moreover, LXA4 was unable to trigger neutrophil upregulation of complement receptor 3 and respiratory burst, and it had no effect on the responses induced by triggering with WKYMVM. We conclude that LXA4 is unable to affect the WKYMVM-induced signalling through FPRL1 and suggest that it acts through a receptor different from FPRL1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/FPR2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyeicosatetraenoic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Lipoxins, http://linkedlifedata.com/resource/pubmed/chemical/Macrophage-1 Antigen, http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/NADPH Oxidase, http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Formyl Peptide, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lipoxin, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Peptide, http://linkedlifedata.com/resource/pubmed/chemical/Trp-Lys-Tyr-Met-Val-Met, http://linkedlifedata.com/resource/pubmed/chemical/lipoxin A4
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0300-9475
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
470-6
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12410796-Calcium Signaling, pubmed-meshheading:12410796-Enzyme Activation, pubmed-meshheading:12410796-Enzyme Induction, pubmed-meshheading:12410796-HL-60 Cells, pubmed-meshheading:12410796-Humans, pubmed-meshheading:12410796-Hydroxyeicosatetraenoic Acids, pubmed-meshheading:12410796-Lipoxins, pubmed-meshheading:12410796-Macrophage-1 Antigen, pubmed-meshheading:12410796-Mitogen-Activated Protein Kinases, pubmed-meshheading:12410796-NADPH Oxidase, pubmed-meshheading:12410796-Neutrophils, pubmed-meshheading:12410796-Oligopeptides, pubmed-meshheading:12410796-Phagocytes, pubmed-meshheading:12410796-Receptors, Cell Surface, pubmed-meshheading:12410796-Receptors, Formyl Peptide, pubmed-meshheading:12410796-Receptors, Immunologic, pubmed-meshheading:12410796-Receptors, Lipoxin, pubmed-meshheading:12410796-Receptors, Peptide, pubmed-meshheading:12410796-Transfection
pubmed:year
2002
pubmed:articleTitle
Phagocyte activation by Trp-Lys-Tyr-Met-Val-Met, acting through FPRL1/LXA4R, is not affected by lipoxin A4.
pubmed:affiliation
DRCD/BBSI (UMR 5092, CEA/CNRS/UJF), Grenoble, Cedex, France.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't