Source:http://linkedlifedata.com/resource/pubmed/id/12406891
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2003-2-13
|
| pubmed:abstractText |
Immunodeficiency following autologous CD34+-purified peripheral blood stem cell (PBSC) transplantation could be related to T-cell depletion of the graft or impaired T-cell reconstitution due to thymus irradiation. Aiming to assess the role of irradiated thymus in T-cell repopulation, we studied 32 adults with multiple myeloma, randomly assigned to receive high-dose therapy including total body irradiation (TBI) followed by autologous transplantation with either unselected or CD34+-selected PBSCs. The median number of reinfused CD3+ cells was lower in the selected group (0.03 versus 14 x 10(6)/kg; P =.002). Lymphocyte subset counts were evaluated from month 3 to 24 after grafting. Naive CD4+ T cells were characterized both by phenotype and by quantification of T-cell receptor rearrangement excision circles (TRECs). The reconstitution of CD3+ and CD4+ T cells was significantly delayed in the CD34+-selected group, but eventually led to counts similar to those found in the unselected group after month 12. Mechanism of reconstitution differed, however, between both groups. Indeed, a marked increase in the naive CD62L+CD45RA+CD4+ subset was observed in the selected group, but not in the unselected group in which half of the CD45RA+CD4+ T cells appear to be CD62L-. Age was identified as an independent adverse factor for CD4+ and CD62L+CD45RA+CD4+ T-cell reconstitution. Our results provide evidence that infusing PBSCs depleted of T cells after TBI in adults delays T-cell reconstitution but accelerates thymic regeneration.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0006-4971
|
| pubmed:author |
pubmed-author:AltesHester KorthalsHK,
pubmed-author:AutranBrigitteB,
pubmed-author:BrouetJean-ClaudeJC,
pubmed-author:CarcelainGuislaineG,
pubmed-author:DebréPatriceP,
pubmed-author:FermandJean-PaulJP,
pubmed-author:LeblondVéroniqueV,
pubmed-author:MalphettesMarionM,
pubmed-author:MarolleauJean-PierreJP,
pubmed-author:Saint-MezardPierreP
|
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
101
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1891-7
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:12406891-Adult,
pubmed-meshheading:12406891-Age Factors,
pubmed-meshheading:12406891-Antigens, CD34,
pubmed-meshheading:12406891-Antigens, CD45,
pubmed-meshheading:12406891-Antineoplastic Combined Chemotherapy Protocols,
pubmed-meshheading:12406891-Cell Separation,
pubmed-meshheading:12406891-Cell Survival,
pubmed-meshheading:12406891-Female,
pubmed-meshheading:12406891-Gene Rearrangement, T-Lymphocyte,
pubmed-meshheading:12406891-Hematopoiesis,
pubmed-meshheading:12406891-Humans,
pubmed-meshheading:12406891-Immunophenotyping,
pubmed-meshheading:12406891-L-Selectin,
pubmed-meshheading:12406891-Lymphocyte Count,
pubmed-meshheading:12406891-Lymphocyte Depletion,
pubmed-meshheading:12406891-Male,
pubmed-meshheading:12406891-Middle Aged,
pubmed-meshheading:12406891-Multiple Myeloma,
pubmed-meshheading:12406891-Peripheral Blood Stem Cell Transplantation,
pubmed-meshheading:12406891-Regeneration,
pubmed-meshheading:12406891-T-Lymphocyte Subsets,
pubmed-meshheading:12406891-Thymus Gland,
pubmed-meshheading:12406891-Transplantation, Autologous,
pubmed-meshheading:12406891-Transplantation Conditioning,
pubmed-meshheading:12406891-Whole-Body Irradiation
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Evidence for naive T-cell repopulation despite thymus irradiation after autologous transplantation in adults with multiple myeloma: role of ex vivo CD34+ selection and age.
|
| pubmed:affiliation |
Laboratoire d'Immunologie Cellulaire et Tissulaire URA CNRS 625, Hôpital de la Pitié-Salpêtrière, Paris, France.
|
| pubmed:publicationType |
Journal Article,
Clinical Trial,
Comparative Study,
Randomized Controlled Trial,
Research Support, Non-U.S. Gov't,
Multicenter Study
|