Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2002-10-30
pubmed:abstractText
Gangliosides, sialic acid-containing glycosphingolipids, are membrane constituents of vertebrates and are known to have important roles in cellular differentiation, adhesion, and recognition. We report here the isolation of a bacterium capable of degrading gangliotetraose-series gangliosides and a new method for the production of glucosylceramide with this bacterium. GM1a ganglioside was found to be sequentially degraded by Paenibacillus sp. strain TS12, which was isolated from soil, as follows: GM1a --> asialo GM1 --> asialo GM2 --> lactosylceramide --> glucosylceramide. TS12 was found to produce a series of ganglioside-degrading enzymes, such as sialidases, beta-galactosidases, and beta-hexosaminidases. TS12 also produced beta-glucosidases, but glucosylceramide was somewhat resistant to the bacterial enzyme under the conditions used. Taking advantage of the specificity, we developed a new method for the production of glucosylceramide using TS12 as a biocatalyst. The method involves the conversion of crude bovine brain gangliosides to glucosylceramide by coculture with TS12 and purification of the product by chromatography with Wakogel C-300 HG.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-10419477, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-10467178, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-10744663, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-10944523, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-1280147, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-13069461, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-13346373, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-13369512, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-1987326, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-2277039, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-2666416, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-3447015, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-368799, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-3771534, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-6084960, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-658424, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-6619111, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-7592649, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-9143118, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-9261060, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-9840984, http://linkedlifedata.com/resource/pubmed/commentcorrection/12406710-9847171
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5241-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Utilization of ganglioside-degrading Paenibacillus sp. strain TS12 for production of glucosylceramide.
pubmed:affiliation
Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't