Source:http://linkedlifedata.com/resource/pubmed/id/12405961
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2002-10-30
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| pubmed:abstractText |
High threshold noxious heat-activated currents and vanilloid receptor-like protein-1 expression were studied in rat cultured primary sensory neurons to find out the molecule(s) responsible for high threshold noxious heat-sensitivity. The average temperature threshold and amplitude of high threshold noxious heat-activated currents were 51.6 +/- 0.13 degrees C and -2.0 +/- 0.1nA (at a holding potential of -60 mV), respectively. The current-voltage relationship of high threshold noxious heat-activated currents was linear at positive membrane potentials, while it showed a weak inward rectification at negative membrane potentials. The average reversal potential measured in control intracellular and extracellular solutions was 4.5 +/- 0.9 mV (n = 6). Ionic substitutions revealed that the high threshold noxious heat-activated current is a nonselective cationic current with calculated ionic permeabilities of Cs+ : Na+ : Ca2+ (1 : 1.3 : 4.5). Consecutive stimuli reduced the heat threshold from 52.2 +/- 1 to 48.4 +/- 1.4 degrees C and then to 44 +/- 0.7 degrees C (n = 3). High threshold noxious heat-activated currents could dose-dependently and reversibly be reduced by ruthenium red (100 nm-10 micro m) but not by capsazepine (10 micro m). The average longest diameter of high threshold noxious heat-sensitive neurons was 31.48 +/- 0.5 micro m (A = approximately 778 micro m2; n = 77). Twenty-three percent of the total neuronal population expressed vanilloid receptor-like protein-1. The average area of the vanilloid receptor-like protein-1-immunopositive cells was 1,696 +/- 65.3 micro m2 (d = approximately 46 micro m). Vanilloid receptor-like protein-1-expressing neurons did not express the vanilloid receptor 1. Comparison of our data with results obtained in vanilloid receptor-like protein-1-expressing non-neuronal cells and previous immunohistochemical findings suggests that high threshold noxious heat-activated currents are produced by vanilloid receptor-like protein-1 and that high threshold heat-sensitive dorsal root ganglion neurons are the perikarya of type I noxious heat-sensitive fibers.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Capsaicin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Drug,
http://linkedlifedata.com/resource/pubmed/chemical/Ruthenium Red,
http://linkedlifedata.com/resource/pubmed/chemical/TRPV Cation Channels,
http://linkedlifedata.com/resource/pubmed/chemical/capsazepine,
http://linkedlifedata.com/resource/pubmed/chemical/vanilloid receptor subtype 1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0953-816X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
16
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1483-9
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:12405961-Afferent Pathways,
pubmed-meshheading:12405961-Animals,
pubmed-meshheading:12405961-Calcium Signaling,
pubmed-meshheading:12405961-Capsaicin,
pubmed-meshheading:12405961-Cell Size,
pubmed-meshheading:12405961-Cells, Cultured,
pubmed-meshheading:12405961-Dose-Response Relationship, Drug,
pubmed-meshheading:12405961-Ganglia, Spinal,
pubmed-meshheading:12405961-Hot Temperature,
pubmed-meshheading:12405961-Immunohistochemistry,
pubmed-meshheading:12405961-Male,
pubmed-meshheading:12405961-Membrane Potentials,
pubmed-meshheading:12405961-Neurons, Afferent,
pubmed-meshheading:12405961-Nociceptors,
pubmed-meshheading:12405961-Pain,
pubmed-meshheading:12405961-Pain Threshold,
pubmed-meshheading:12405961-Rats,
pubmed-meshheading:12405961-Rats, Sprague-Dawley,
pubmed-meshheading:12405961-Receptors, Drug,
pubmed-meshheading:12405961-Ruthenium Red,
pubmed-meshheading:12405961-Synaptic Transmission,
pubmed-meshheading:12405961-TRPV Cation Channels,
pubmed-meshheading:12405961-Thermosensing
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| pubmed:year |
2002
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| pubmed:articleTitle |
The putative role of vanilloid receptor-like protein-1 in mediating high threshold noxious heat-sensitivity in rat cultured primary sensory neurons.
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| pubmed:affiliation |
Department of Anaesthetics and Intensive Care, Imperial College, Faculty of Medicine, Chelsea and Westminster Hospital, 369 Fulham Road, London, SW10 9NH, UK.
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| pubmed:publicationType |
Journal Article
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