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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
44
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pubmed:dateCreated |
2002-10-30
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pubmed:abstractText |
Typical eukaryotic transcriptional activators are composed of distinct functional domains, including a DNA binding domain and an activating domain. Artificial transcription factors have been designed wherein the DNA binding domain is a minor groove DNA binding hairpin polyamide linked by a flexible tether to short activating peptides, typically 16-20 residues in size. In this study, the linker between the polyamide and the peptide was altered in an incremental fashion using rigid oligoproline "molecular rulers" in the 18-45 A length range. We find that there is an optimal linker length which separates the DNA and the activation region for transcription activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0002-7863
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
124
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13067-71
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12405833-Amino Acid Sequence,
pubmed-meshheading:12405833-Base Sequence,
pubmed-meshheading:12405833-Biomimetic Materials,
pubmed-meshheading:12405833-Cross-Linking Reagents,
pubmed-meshheading:12405833-DNA-Binding Proteins,
pubmed-meshheading:12405833-Models, Molecular,
pubmed-meshheading:12405833-Molecular Sequence Data,
pubmed-meshheading:12405833-Peptides,
pubmed-meshheading:12405833-Protein Conformation,
pubmed-meshheading:12405833-Protein Structure, Tertiary,
pubmed-meshheading:12405833-Structure-Activity Relationship,
pubmed-meshheading:12405833-Transcription Factors,
pubmed-meshheading:12405833-Transcriptional Activation
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pubmed:year |
2002
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pubmed:articleTitle |
Design of artificial transcriptional activators with rigid poly-L-proline linkers.
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pubmed:affiliation |
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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