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rdf:type |
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lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0013879,
umls-concept:C0017349,
umls-concept:C0020792,
umls-concept:C0034987,
umls-concept:C0035820,
umls-concept:C0040627,
umls-concept:C0086860,
umls-concept:C0162493,
umls-concept:C0205147,
umls-concept:C0205314,
umls-concept:C0214635,
umls-concept:C0242210,
umls-concept:C0679622,
umls-concept:C1158770,
umls-concept:C1824738
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pubmed:issue |
9
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pubmed:dateCreated |
2002-10-22
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pubmed:abstractText |
The class II trans-activator (CIITA), which acts as a master regulator for expression of MHC class II genes, is expressed constitutively in mature B cells. This constitutive expression of CIITA is driven by CIITA promoter III (CIITA-PIII). However, little is known about the factors that control the B cell-mediated trans-activation of CIITA-PIII. In this study using B cells we have identified several cAMP-responsive elements (CREs) in the proximal promoter and in the 5'-untranslated region (5'-UTR) that are involved in the activation of CIITA-PIII. We show that activating transcription factor (ATF)/CRE binding protein (CREB) factors bind to the CREs in vitro and in vivo. Notably, our results also reveal that the 5'-UTR of CIITA-PIII functions as an important regulatory region in B lymphocytes. Furthermore, transient cotransfections of a CIITA-PIII luciferase reporter construct with either CREB-1 or ATF-1 boost CIITA-PIII trans-activation in a dose-dependent manner, which was further enhanced by addition of general coactivator CREB-binding protein. Transient transfections using mutant CIITA-PIII luciferase reporter constructs that either lack the (5'-UTR) or abolish binding of CREB-1 and ATF-1 to the CRE located in activation response element-2, displayed severely reduced promoter activity in B cells. A similar successive deletion of the CREs resulted in a subsequent reduction of CREB-1-induced activity of CIITA-PIII in B cells. Together our results argue for an important role of ATF/CREB factors and the 5'-UTR of CIITA-PIII in the trans-activation of CIITA-PIII in B cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/CREB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response Element...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MHC class II transactivator protein,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5061-71
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12391222-5' Untranslated Regions,
pubmed-meshheading:12391222-Activating Transcription Factor 1,
pubmed-meshheading:12391222-B-Lymphocyte Subsets,
pubmed-meshheading:12391222-Cell Line, Transformed,
pubmed-meshheading:12391222-Cyclic AMP,
pubmed-meshheading:12391222-Cyclic AMP Response Element Modulator,
pubmed-meshheading:12391222-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:12391222-DNA-Binding Proteins,
pubmed-meshheading:12391222-Genes, MHC Class II,
pubmed-meshheading:12391222-HeLa Cells,
pubmed-meshheading:12391222-Humans,
pubmed-meshheading:12391222-Jurkat Cells,
pubmed-meshheading:12391222-Mutagenesis, Site-Directed,
pubmed-meshheading:12391222-Nuclear Proteins,
pubmed-meshheading:12391222-Promoter Regions, Genetic,
pubmed-meshheading:12391222-Protein Binding,
pubmed-meshheading:12391222-Repressor Proteins,
pubmed-meshheading:12391222-Response Elements,
pubmed-meshheading:12391222-Sequence Deletion,
pubmed-meshheading:12391222-Trans-Activators,
pubmed-meshheading:12391222-Transcription, Genetic,
pubmed-meshheading:12391222-Transcription Factors,
pubmed-meshheading:12391222-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Transcriptional regulation of the MHC class II trans-activator (CIITA) promoter III: identification of a novel regulatory region in the 5'-untranslated region and an important role for cAMP-responsive element binding protein 1 and activating transcription factor-1 in CIITA-promoter III transcriptional activation in B lymphocytes.
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pubmed:affiliation |
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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