Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-10-22
pubmed:abstractText
Fragments of ileum from 663 pigs were collected in abattoirs, prepared with the use of standard histological methods and stained with a novel sensitive histochemical method for the detection of porcine proliferative enteropathy. The method is a combination of the following 3 well-known methods, the Warthin-Starry method, alcian blue and hematoxylin-eosin. In 11 cases, mucus-producing cells were completely absent, severe adenomatous proliferation was observed and intracellular bacteria were found in enterocytes. Disappearance of goblet cells and the presence of adenomatous proliferation without any detectable intracellular bacteria were observed in 16 cases. In the remaining 636 cases, histological changes and intracellular bacteria were not found. When comparing the conventional Warthin-Starry method with the modified staining method presented here, the same 16 cases were found. However, the method presented here enables examination of large numbers of sections in a relatively short period of time.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0065-1281
pubmed:author
pubmed:issnType
Print
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
285-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Silver staining combined with alcian blue and hematoxylin-eosin for the detection of Lawsonia intracelullaris in swine proliferative enteropathy.
pubmed:affiliation
Department of Veterinary Pathology, UFRGS, Porto Alegre, RS, Brazil. davetpat@vortex.ufrgs.br
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't