Linked Life Data

12388170

Source:http://linkedlifedata.com/resource/pubmed/id/12388170

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-12-17
pubmed:abstractText
The high activity of the insulin-signaling pathway contributes to the enhanced feeding-induced stimulation of translation initiation in skeletal muscle of neonatal pigs. Protein-tyrosine-phosphatase 1B (PTP1B) is a negative regulator of the tyrosine phosphorylation of the insulin receptor (IR) and insulin receptor substrate 1 (IRS-1). The activity of PTP1B is determined mainly by its association with IR and Grb2. We examined the level of PTP1B activity, PTP1B protein abundance, PTP1B tyrosine phosphorylation, and the association of PTP1B with IR and Grb2 in skeletal muscle and liver of fasted and fed 7- and 26-day-old pigs. PTP1B activity in skeletal muscle was lower (P < 0.05) in 7- compared with 26-day-old pigs but in liver was similar in the two age groups. PTP1B abundances were similar in muscle but lower (P < 0.05) in liver of 7- compared with 26-day-old pigs. PTP1B tyrosine phosphorylation in muscle was lower (P < 0.05) in 7- than in 26-day-old pigs. The associations of PTP1B with IR and with Grb2 were lower (P < 0.05) at 7 than at 26 days of age in muscle, but there were no age effects in liver. Finally, in both age groups, fasting did not have any effect on these parameters. These results indicate that basal PTP1B activation is developmentally regulated in skeletal muscle of neonatal pigs, consistent with the developmental changes in the activation of the insulin-signaling pathway reported previously. Reduced PTP1B activation in neonatal muscle likely contributes to the enhanced insulin sensitivity of skeletal muscle in neonatal pigs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0193-1849
pubmed:author
pubmed:issnType
Print
pubmed:volume
284
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
E47-54
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:12388170-Adaptor Proteins, Signal Transducing, pubmed-meshheading:12388170-Animals, pubmed-meshheading:12388170-Animals, Newborn, pubmed-meshheading:12388170-Blotting, Western, pubmed-meshheading:12388170-Carrier Proteins, pubmed-meshheading:12388170-Enzyme Activation, pubmed-meshheading:12388170-Fasting, pubmed-meshheading:12388170-Food, pubmed-meshheading:12388170-Immunosorbent Techniques, pubmed-meshheading:12388170-Insulin, pubmed-meshheading:12388170-Insulin Receptor Substrate Proteins, pubmed-meshheading:12388170-Muscle, Skeletal, pubmed-meshheading:12388170-Phosphoproteins, pubmed-meshheading:12388170-Phosphorylation, pubmed-meshheading:12388170-Phosphotyrosine, pubmed-meshheading:12388170-Protein Tyrosine Phosphatase, Non-Receptor Type 1, pubmed-meshheading:12388170-Protein Tyrosine Phosphatases, pubmed-meshheading:12388170-Receptor, Insulin, pubmed-meshheading:12388170-Signal Transduction, pubmed-meshheading:12388170-Swine
pubmed:year
2003
pubmed:articleTitle
Protein-tyrosine-phosphatase 1B activation is regulated developmentally in muscle of neonatal pigs.
pubmed:affiliation
United States Department of Agriculture/Agriculture Research Service, Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.