Source:http://linkedlifedata.com/resource/pubmed/id/12382018
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2002-10-16
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| pubmed:abstractText |
Human high-risk papillomaviruses (HR-HPVs) are involved in the induction of invasive cervical cancer. The aim of this study was to introduce a simple, semi-automated and reproducible approach suitable for HR-HPV detection in clinical practice. The procedure is based on DNA isolation, nested polymerase chain reaction, single strand conformational polymorphism and evaluation of HR-HPV genotypes with Gel-Pro software. The clinical performance of the new approach was assessed in two different patient materials: 1) cervical smears with cytological classification Pap2-3 or Pap3 lacking nuclear atypia (anisonucleosis and polychromasia) or koilocytotic atypia and without any previous therapy 2) formalin-fixed, paraffin-embedded cervical carcinoma and lymph node sections. Using the new approach we detected HR-HPV DNA in 64% patient samples cytologically classified as Pap2-3 or Pap3 respectively and in 80% formalin-fixed, paraffin-embedded lymph node sections histologically classified as lymph nodes without carcinoma cell infiltration. The combination of methods described in this study results in increased sensitivity of HR-HPV identification allowing detection of HPV DNA in a very small amount of target DNA so that it can be widely used in distinguishing the pre- malignant lesions and in determination of invading carcinoma cells to lymph nodes in patients with advanced cervical cancer. The new approach is useful in unambiguous HR-HPV genotyping even in double-HPV infection.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
0028-2685
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
49
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
217-24
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12382018-Automation,
pubmed-meshheading:12382018-Carcinoma,
pubmed-meshheading:12382018-Cervix Uteri,
pubmed-meshheading:12382018-DNA, Viral,
pubmed-meshheading:12382018-Female,
pubmed-meshheading:12382018-Genotype,
pubmed-meshheading:12382018-Humans,
pubmed-meshheading:12382018-Lymphatic Metastasis,
pubmed-meshheading:12382018-Papillomaviridae,
pubmed-meshheading:12382018-Papillomavirus Infections,
pubmed-meshheading:12382018-Polymerase Chain Reaction,
pubmed-meshheading:12382018-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:12382018-Precancerous Conditions,
pubmed-meshheading:12382018-Sensitivity and Specificity,
pubmed-meshheading:12382018-Software,
pubmed-meshheading:12382018-Tumor Virus Infections,
pubmed-meshheading:12382018-Uterine Cervical Neoplasms
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| pubmed:year |
2002
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| pubmed:articleTitle |
New approach to human high-risk papillomavirus (HR-HPV) genotyping.
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| pubmed:affiliation |
Department of Clinical Genetics, St. Elizabeth Cancer Institute, 812 50 Bratislava 1, Slovak Republic. eweisman@ousa.sk
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| pubmed:publicationType |
Journal Article
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