Source:http://linkedlifedata.com/resource/pubmed/id/12380643
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2002-10-16
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| pubmed:abstractText |
Capping and release of membranous, small (< 1.5 microm) endothelial microparticles were quantified by immunofluorescence microscopy and flow cytometry after treatment of cultures of human renal microvascular endothelial cells with agonists tumor necrosis factor-alpha (TNF-alpha) or mitomycin C. For constitutive marker CD31, both agonist-treated attached, monolayer, and detached, free endothelial cells formed caps and released microparticles. TNF-alpha and mitomycin C induced dissimilar appearing CD31-containing caps after 3 h, followed by endothelial microparticle release after 6 h. The degree of capping correlated with increasing counts of released microparticles. For lymphokine-inducible CD54, TNF-alpha also induced CD54-containing caps and microparticle release, but mitomycin C failed to induce the expression of either entity. Neither capping nor microparticle release caused by TNF-alpha was part of an apoptotic pathway that involved caspase 3. Mitomycin C treatment of endothelial cells caused capping and microparticle release with a time course similar to TNF-alpha induction for 15 to 24 h, but assays for caspase 3 were positive, confirming the apoptotic action of mitomycin C. Membrane capping and microparticle release from endothelial cells are a convenient experimental model for studying protein movement, release of microparticles, and their possible biological significance.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD31,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1062-3329
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
179-89
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12380643-Antibodies, Monoclonal,
pubmed-meshheading:12380643-Antigens, CD31,
pubmed-meshheading:12380643-Apoptosis,
pubmed-meshheading:12380643-Caspase 3,
pubmed-meshheading:12380643-Caspases,
pubmed-meshheading:12380643-Cell Adhesion Molecules,
pubmed-meshheading:12380643-Cell Extracts,
pubmed-meshheading:12380643-Cells, Cultured,
pubmed-meshheading:12380643-Endothelium, Vascular,
pubmed-meshheading:12380643-Flow Cytometry,
pubmed-meshheading:12380643-Humans,
pubmed-meshheading:12380643-Intercellular Adhesion Molecule-1,
pubmed-meshheading:12380643-Kidney,
pubmed-meshheading:12380643-Microcirculation,
pubmed-meshheading:12380643-Microscopy, Fluorescence,
pubmed-meshheading:12380643-Microspheres,
pubmed-meshheading:12380643-Mitomycin,
pubmed-meshheading:12380643-Protein Transport,
pubmed-meshheading:12380643-Tumor Necrosis Factor-alpha
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Agonist-induced capping of adhesion proteins and microparticle shedding in cultures of human renal microvascular endothelial cells.
|
| pubmed:affiliation |
Department of Medicine, University of Miami School of Medicine, Florida 33101, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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