Source:http://linkedlifedata.com/resource/pubmed/id/12370361
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2002-10-8
|
| pubmed:abstractText |
Isotype switching by murine B cells follows a pattern whereby the proportion of cells undergoing switching increases with division number and is regulated by cytokines. Here we explored whether human B cells behaved in a similar manner. The effect of IL-4, IL-10, and IL-13, alone or in combination, on Ig isotype switching by highly purified naive human CD40 ligand (CD40L)-activated B cells was measured against division number over various harvest times. Switching to IgG was induced by IL-4 and, to a lesser extent, IL-13 and IL-10. The combination of IL-10 with IL-4, but not IL-13, induced a higher percentage of cells to undergo switching. Isotype switching to IgG by human CD40L-activated naive B cells was found to be linked to the division history of the cells: IgG(+) cells appeared in cultures of B cells stimulated with CD40L and IL-4 after approximately the third cell division, with the majority expressing IgG1, thus revealing a predictable pattern of IgG isotype switching. These results reveal a useful quantitative framework for monitoring the effects of cytokines on proliferation and isotype switching that should prove valuable for screening Ig immunodeficiencies and polymorphisms in the population for a better understanding of the regulation of human humoral immune responses.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin D,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
|
| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
169
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
4298-306
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12370361-B-Lymphocytes,
pubmed-meshheading:12370361-CD40 Ligand,
pubmed-meshheading:12370361-Cell Division,
pubmed-meshheading:12370361-Cell Separation,
pubmed-meshheading:12370361-Cells, Cultured,
pubmed-meshheading:12370361-Cytokines,
pubmed-meshheading:12370361-Down-Regulation,
pubmed-meshheading:12370361-Fetal Blood,
pubmed-meshheading:12370361-Humans,
pubmed-meshheading:12370361-Immunoglobulin Class Switching,
pubmed-meshheading:12370361-Immunoglobulin D,
pubmed-meshheading:12370361-Immunoglobulin G,
pubmed-meshheading:12370361-Immunoglobulin M,
pubmed-meshheading:12370361-Immunoglobulins,
pubmed-meshheading:12370361-Interleukin-10,
pubmed-meshheading:12370361-Interleukin-13,
pubmed-meshheading:12370361-Interleukin-4,
pubmed-meshheading:12370361-Interphase,
pubmed-meshheading:12370361-Lymphocyte Activation,
pubmed-meshheading:12370361-Spleen,
pubmed-meshheading:12370361-Time Factors
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| pubmed:year |
2002
|
| pubmed:articleTitle |
Isotype switching by human B cells is division-associated and regulated by cytokines.
|
| pubmed:affiliation |
Immune Regulation Group, Centenary Institute for Cancer Medicine and Cell Biology, Locked Bag #6, Newtown, New South Wales 2042, Australia. s.tangye@centrenary.usyd.edu.au
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|