Linked Life Data

12242716

Source:http://linkedlifedata.com/resource/pubmed/id/12242716

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-9-20
pubmed:abstractText
The molecular mechanisms of pulmonary vascular development are poorly understood. Cell-specific developmental pathways are influenced by cell-cell signaling. Notch signaling molecules are highly conserved receptors active in many cell-fate determination systems. Recent observations of Notch molecules and a Notch ligand, Jagged1, suggest their importance in vascular morphogenesis, and particularly pulmonary vascular development. We performed a systematic evaluation of Notch1/Jagged1 gene and protein expression in the developing mouse lung from embryonic day 11 until adulthood by using quantitative PCR, immunofluorescence, and electron microscopic analysis. mRNA transcripts for Notch1-4 and Jagged1 increased progressively from early to later lung development, accompanied by a simultaneous rise in endothelial cell-specific gene expression, a pattern not seen in other organs. Notch1 mRNA was identified on both epithelial and mesenchymal structures of the embryonic lung. Immunofluorescence staining revealed the progressive acquisition of Notch1 and Jagged1 proteins by the emerging endothelium. Notch1 and Jagged1 were seen initially on well-formed, larger vessels within the embryonic lung bud and progressively on finer vascular networks. Each was also expressed on surrounding nonvascular structures. The localization of Notch1 and Jagged1 on endothelial cell surface membranes within the alveolar microvasculature was confirmed by immuno-electron microscopy. These temporal and spatial patterns in Notch1/Jagged1 gene and protein expression suggest multiple potential paths of cell-cell signaling during lung development and vascular morphogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1058-8388
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
225
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
166-75
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12242716-Animals, pubmed-meshheading:12242716-Brain, pubmed-meshheading:12242716-Calcium-Binding Proteins, pubmed-meshheading:12242716-Cell Membrane, pubmed-meshheading:12242716-Endothelium, Vascular, pubmed-meshheading:12242716-Immunohistochemistry, pubmed-meshheading:12242716-In Situ Hybridization, pubmed-meshheading:12242716-Intercellular Signaling Peptides and Proteins, pubmed-meshheading:12242716-Kidney, pubmed-meshheading:12242716-Lung, pubmed-meshheading:12242716-Membrane Proteins, pubmed-meshheading:12242716-Mice, pubmed-meshheading:12242716-Microscopy, Electron, pubmed-meshheading:12242716-Microscopy, Fluorescence, pubmed-meshheading:12242716-Protein Biosynthesis, pubmed-meshheading:12242716-Proteins, pubmed-meshheading:12242716-RNA, Messenger, pubmed-meshheading:12242716-Receptor, Notch1, pubmed-meshheading:12242716-Receptors, Cell Surface, pubmed-meshheading:12242716-Receptors, Notch, pubmed-meshheading:12242716-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12242716-Signal Transduction, pubmed-meshheading:12242716-Time Factors, pubmed-meshheading:12242716-Transcription Factors
pubmed:year
2002
pubmed:articleTitle
Notch1 and Jagged1 expression by the developing pulmonary vasculature.
pubmed:affiliation
Pulmonary, Allergy and Critical Care Division, University of Pennsylvania Medical Center, Children's Hospital of Philadelphia and the University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA. dtaichma@mail.med.upenn.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.