Linked Life Data

12237400

Source:http://linkedlifedata.com/resource/pubmed/id/12237400

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2002-10-2
pubmed:abstractText
Gene expression is a stochastic, or "noisy," process. This noise comes about in two ways. The inherent stochasticity of biochemical processes such as transcription and translation generates "intrinsic" noise. In addition, fluctuations in the amounts or states of other cellular components lead indirectly to variation in the expression of a particular gene and thus represent "extrinsic" noise. Here, we show how the total variation in the level of expression of a given gene can be decomposed into its intrinsic and extrinsic components. We demonstrate theoretically that simultaneous measurement of two identical genes per cell enables discrimination of these two types of noise. Analytic expressions for intrinsic noise are given for a model that involves all the major steps in transcription and translation. These expressions give the sensitivity to various parameters, quantify the deviation from Poisson statistics, and provide a way of fitting experiment. Transcription dominates the intrinsic noise when the average number of proteins made per mRNA transcript is greater than approximately 2. Below this number, translational effects also become important. Gene replication and cell division, included in the model, cause protein numbers to tend to a limit cycle. We calculate a general form for the extrinsic noise and illustrate it with the particular case of a single fluctuating extrinsic variable-a repressor protein, which acts on the gene of interest. All results are confirmed by stochastic simulation using plausible parameters for Escherichia coli.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-10098409, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-10681449, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-10850721, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-11062240, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-11388089, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-11438714, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-11720979, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-11967532, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-12183631, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-1787735, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-2403552, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-8526893, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-9023339, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-958399, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-96307, http://linkedlifedata.com/resource/pubmed/commentcorrection/12237400-9691025
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12795-800
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Intrinsic and extrinsic contributions to stochasticity in gene expression.
pubmed:affiliation
Center for Studies in Physics and Biology and Laboratory for Cancer Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. swain@cnd.mcgill.ca
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't