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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
2002-9-10
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pubmed:abstractText |
Insulin-like growth factor binding protein-3, IGFBP-3, specifically binds to IGFs with high affinity, but it is also capable of modulating the IGF-I signalling pathway or inducing apoptosis independently of its binding to IGFs. The molecular mechanisms underlying the action of IGFBP-3 have not been elucidated. In this study, we have demonstrated that binding of IGFBP-3 to a cell surface receptor in MCF-7 breast carcinoma cells induces a rapid and transient increase in intracellular free calcium. This increase was mediated via a pertussis toxin-sensitive pathway, indicating that the IGFBP-3 receptor may be specifically coupled to a Gi protein. The effect of IGFBP-3 on calcium concentrations was dose-dependent and also occurred when IGFBP-3 was complexed with either IGF-I or heparin, suggesting that the receptor binding site is probably located in the least conserved central domain of IGFBP-3. Neither IGFBP-1, nor IGFBP-5 (structurally the closest to IGFBP-3) altered intracellular calcium concentrations. These results provide evidence that a specific intracellular signal is triggered by IGFBP-3 binding to a cell surface receptor.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
527
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
293-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12220677-Breast Neoplasms,
pubmed-meshheading:12220677-Calcium,
pubmed-meshheading:12220677-Carcinoma,
pubmed-meshheading:12220677-Dose-Response Relationship, Drug,
pubmed-meshheading:12220677-Female,
pubmed-meshheading:12220677-Heparin,
pubmed-meshheading:12220677-Humans,
pubmed-meshheading:12220677-Insulin-Like Growth Factor Binding Protein 1,
pubmed-meshheading:12220677-Insulin-Like Growth Factor Binding Protein 3,
pubmed-meshheading:12220677-Insulin-Like Growth Factor Binding Protein 5,
pubmed-meshheading:12220677-Insulin-Like Growth Factor I,
pubmed-meshheading:12220677-Pertussis Toxin,
pubmed-meshheading:12220677-Receptors, Cell Surface,
pubmed-meshheading:12220677-Thapsigargin,
pubmed-meshheading:12220677-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Insulin-like growth factor binding protein-3 increases intracellular calcium concentrations in MCF-7 breast carcinoma cells.
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pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale, Unité 515, Hôpital Saint-Antoine, 184 rue du Fbg St-Antoine, 75012, Paris, France. ricort@st-antoine.inserm.fr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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