Linked Life Data

12211070

Source:http://linkedlifedata.com/resource/pubmed/id/12211070

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-9-4
pubmed:abstractText
Ran, a Ras-related GTPase, is required for transporting proteins in and out of the nucleus during interphase and for regulating the assembly of microtubules. cDNA cloning shows that rat testis, like mouse testis, expresses both somatic and testis-specific forms of Ran-GTPase. The presence of a homologous testis-specific form of Ran-GTPase in rodents implies that the Ran-GTPase pathway plays a significant role during sperm development. This suggestions is supported by distinct Ran-GTPase immunolocalization sites identified in developing spermatids. Confocal microscopy demonstrates that Ran-GTPase localizes in the nucleus of round spermatids and along the microtubules of the manchette in elongating spermatids. When the manchette disassembles, Ran-GTPase immunoreactivity is visualized in the centrosome region of maturing spermatids. The circumstantial observation that fractionated manchettes, containing copurified centrin-immunoreactive centrosomes, can organize a three-dimensional lattice in the presence of taxol and GTP, points to the role of Ran-GTPase and associated factors in microtubule nucleation as well as the potential nucleating function of spermatid centrosomes undergoing a reduction process. Electron microscopy demonstrates the presence in manchette preparations of spermatid centrosomes, recognized as such by their association with remnants of the implantation fossa, a dense plate observed only at the basal surface of developing spermatid and sperm nuclei. In addition, we have found importin beta1 immunoreactivity in the nucleus of elongating spermatids, a finding that, together with the presence of Ran-GTPase in the nucleus of round spermatids and the manchette, suggest a potential role of Ran-GTPase machinery in nucleocytoplasmic transport. Our expression and localization analysis, correlated with functional observations in other cell systems, suggest that Ran-GTPase may be involved in both nucleocytoplasmic transport and microtubules assembly, two critical events during the development of functional sperm. In addition, the manchette-to-centrosome Ran-GTPase relocation, together with the similar redistribution of various proteins associated to the manchette, suggest the existence of an intramanchette molecular transport mechanism, which may share molecular analogies with intraflagellar transport.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1040-452X
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
131-40
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:12211070-Amino Acid Sequence, pubmed-meshheading:12211070-Animals, pubmed-meshheading:12211070-Cell Nucleus, pubmed-meshheading:12211070-Centrosome, pubmed-meshheading:12211070-Cloning, Molecular, pubmed-meshheading:12211070-DNA, Complementary, pubmed-meshheading:12211070-Guanosine Triphosphate, pubmed-meshheading:12211070-Isoenzymes, pubmed-meshheading:12211070-Male, pubmed-meshheading:12211070-Microtubules, pubmed-meshheading:12211070-Molecular Sequence Data, pubmed-meshheading:12211070-Paclitaxel, pubmed-meshheading:12211070-Protein Transport, pubmed-meshheading:12211070-Rats, pubmed-meshheading:12211070-Sequence Alignment, pubmed-meshheading:12211070-Sequence Homology, Amino Acid, pubmed-meshheading:12211070-Sperm Head, pubmed-meshheading:12211070-Spermatogenesis, pubmed-meshheading:12211070-Spermatozoa, pubmed-meshheading:12211070-Testis, pubmed-meshheading:12211070-beta Karyopherins, pubmed-meshheading:12211070-ran GTP-Binding Protein
pubmed:year
2002
pubmed:articleTitle
Ran, a GTP-binding protein involved in nucleocytoplasmic transport and microtubule nucleation, relocates from the manchette to the centrosome region during rat spermiogenesis.
pubmed:affiliation
Department of Cell Biology and Anatomical Sciences, CUNY Medical School, New York, New York 10031, USA. kier@med.cuny.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.