Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
|
pubmed:dateCreated |
1976-6-2
|
pubmed:abstractText |
Sleep-promoting factor (factor S) was extracted, partially purified, and concentrated from cerebrospinal fluid and from acid-acetone extracts of brain stem anc cortex of sleep-deprived goats and sheep. 2. Solutes greater than 500 daltons were largely removed by serial ultrafiltrations through molecular sieves (Amicon membranes UM10 and UM05); solutes less than 350 daltons were largely eliminated by gel filtration through Sephadex G10 columns. Sleep-promoting activity was found in a fraction eluted prior to [14C] sucrose marker. 3. Concentrated fraction were infused intraventricularly in rats (0.1 ml in 30 min just prior to 12-h dark cycle) and in rabbits (0.3 ml in 90 min in morning). Sleep-promoting activity was assayed by decrease in nocturnal locomotor activity of rats and by duration and amplitude of slow-wave cortical EEG in rabbits.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0022-3077
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
38
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1299-311
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:1221075-Animals,
pubmed-meshheading:1221075-Brain,
pubmed-meshheading:1221075-Cerebrospinal Fluid,
pubmed-meshheading:1221075-Electroencephalography,
pubmed-meshheading:1221075-Motor Activity,
pubmed-meshheading:1221075-Pronase,
pubmed-meshheading:1221075-Rabbits,
pubmed-meshheading:1221075-Rats,
pubmed-meshheading:1221075-Sheep,
pubmed-meshheading:1221075-Sleep,
pubmed-meshheading:1221075-Sleep Deprivation
|
pubmed:year |
1975
|
pubmed:articleTitle |
Extraction of sleep-promoting factor S from cerebrospinal fluid and from brains of sleep-deprived animals.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|