Linked Life Data

12209916

Source:http://linkedlifedata.com/resource/pubmed/id/12209916

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-9-4
pubmed:abstractText
Clinically available synthetic ePTFE vascular grafts frequently fail when used for small-diameter arterial substitution. The lower long-term patency of ePTFE grafts is due mainly to thrombogenicity and poor healing. We developed a new small-diameter (1.5-mm) ePTFE vascular prosthesis coated with polyamino-acid urethane copolymer (PAU) to enhance endothelialization. Coating with PAU made the hydrophobic ePTFE vascular graft hydrophilic. Scanning electron microscopy (SEM) observation showed that PAU was homogeneously coated on the ePTFE graft while maintaining the graft's porous structure. Fourier transform infrared attenuated total reflectance (FTIR-ATR) spectra showed PAU was present on graft inner surfaces. Noncoated and PAU-coated ePTFE vascular grafts were implanted into the rat abdominal aorta and evaluated within 1 week and at 8 weeks after implantation. We evaluated the extent of neoendothelialization by SEM, light microscopy, and immunohistochemical staining. Noncoated grafts showed partial endothelialization at proximal and distal areas of grafts but none at midsection. One-percent PAU-coated grafts showed incomplete neoendothelialization, but endothelial cells were observed in the midsection of grafts. Three-percent PAU-coated ePTFE grafts showed complete endothelialization with typical endothelial cell layers under SEM and histologic observation. Immunohistochemical staining also showed that an endothelial cell lining positively reacted with anti-von Willebrand factor (endothelial cell marker) antibody. We conclude that PAU-coated ePTFE vascular grafts enhance endothelialization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9304
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Wiley Periodicals, Inc.
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
A new vascular prosthesis coated with polyamino-acid urethane copolymer (PAU) to enhance endothelialization.
pubmed:affiliation
3-Dimensional Cell & Tissue Engineering Group, National Institute for Advanced Interdisciplinary Research, Agency of Industrial Science and Technology, 1-1-4 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't