Linked Life Data

12193074

Source:http://linkedlifedata.com/resource/pubmed/id/12193074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2002-8-23
pubmed:abstractText
To date various types of Cl(-) currents have been recorded in cardiac myocytes from different regions of the heart and from different species. Most of these are silent under basal conditions, but are rapidly activated under the influence of various agonists or physical stress that, in the long term, also lead to development of hypertrophy. Previously, we identified three different Cl(-) channel activities in neonatal rat cardiomyocytes: (i) Ca(2+) regulated, (ii) cAMP regulated (cystic fibrosis transmembrane conductance regulator Cl(-) channels) and (iii) osmoregulated Cl(-) channels. In this study, we examined comparatively the effects of cyclic stretch and endothelin-1 (ET-1) on Cl(-) channel activity in primary cultures of neonatal rat ventricular myocytes using an (125)I-efflux assay. About 4 min after the start of the (125)I-efflux (mean basal rate amounts 6.3% of total (125)I incorporated/min), the addition of 10 nM ET-1 or the application of cyclic stretch rapidly and transiently increased (125)I-efflux by 3.8%/min and 0.8%/min respectively above the basal rate. The stretch induced (125)I-efflux rate could be blocked by 100 microM Gd(3+) but it had no effect on the ET-1 response. After 24 h stimulation by ET-1 or cyclic stretch the myocytes responded by hypertrophy which is detected by increases of (3)H-leucine incorporation into protein and protein/DNA ratio. In conclusion, cyclic stretch as well as ET rapidly and transiently activate Cl(-) channels in rat neonatal cardiomyocytes. The results suggest that the activation of distinct types of Cl(-) channels (co)transduce the stretch- and agonist-induced hypertrophic responses in these myocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0143-5221
pubmed:author
pubmed:issnType
Print
pubmed:volume
103 Suppl 48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
148S-151S
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Cyclic stretch and endothelin-1 mediated activation of chloride channels in cultured neonatal rat ventricular myocytes.
pubmed:affiliation
Department of Biochemistry, Cardiovascular Research Institute COEUR, Faculty of Medicine and Health Sciences, Erasmus University, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't