Source:http://linkedlifedata.com/resource/pubmed/id/12191517
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
2002-8-22
|
| pubmed:abstractText |
There is a growing interest in using dendritic cells (DC) for vaccine approaches in the treatment of cancer and infectious diseases. This requires a reproducible method for the generation of large numbers of DC in a closed culture system suitable for clinical use and conforming to the current guidelines of good manufacturing practices. We designed a system in which the DC were generated in a closed system from adherent monocytes using Cell Factories (DC-CF). Monocytes were enriched from apheresis products by adherence and then cultured in the presence of AB serum or autologous plasma and GM-CSF and IL-4 for 6 days. The DC generated in Cell Factories were extensively compared to research-grade DC generated in conventional tissue culture flasks (DC-TCF). At day 6, the immature DC were harvested and the yield, the viability, the immunophenotype and the functional characteristics of the DC were compared.DC-CF and DC-TCF showed similar viability and purity and scored equally when tested for stability, dextran and latex bead uptake, in MLR and in the activation of influenza-specific memory cells after electroporation with influenza matrix protein 1 (IMP1) mRNA. These data indicated that large numbers of functional clinical-grade DC could be generated from adherent cells in a closed system using Cell Factories.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-1759
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
264
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
135-51
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| pubmed:dateRevised |
2009-11-3
|
| pubmed:meshHeading |
pubmed-meshheading:12191517-Animals,
pubmed-meshheading:12191517-Cell Adhesion,
pubmed-meshheading:12191517-Cell Count,
pubmed-meshheading:12191517-Cell Culture Techniques,
pubmed-meshheading:12191517-Cell Differentiation,
pubmed-meshheading:12191517-Cell Line,
pubmed-meshheading:12191517-Clone Cells,
pubmed-meshheading:12191517-Cryopreservation,
pubmed-meshheading:12191517-Culture Techniques,
pubmed-meshheading:12191517-Dendritic Cells,
pubmed-meshheading:12191517-Electroporation,
pubmed-meshheading:12191517-Humans,
pubmed-meshheading:12191517-Immunologic Memory,
pubmed-meshheading:12191517-Lymphocyte Activation,
pubmed-meshheading:12191517-Mice,
pubmed-meshheading:12191517-Monocytes,
pubmed-meshheading:12191517-RNA, Messenger,
pubmed-meshheading:12191517-T-Lymphocyte Subsets,
pubmed-meshheading:12191517-Viral Matrix Proteins
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Generation of large numbers of dendritic cells in a closed system using Cell Factories.
|
| pubmed:affiliation |
Laboratory of Molecular and Cellular Therapy, Department of Physiology-Immunology, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103/E, Brussels, Belgium.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|