Source:http://linkedlifedata.com/resource/pubmed/id/12181198
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2002-8-15
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| pubmed:abstractText |
Insulin-like growth factor I (IGF-I) and transforming growth factor-beta1 (TGF-beta1) are upregulated in myofibroblasts at sites of fibrosis in experimental enterocolitis and in Crohn's disease (CD). We compared the sites of expression of IGF-I and TGF-beta1 in a rat peptidoglycan-polysaccharide (PG-PS) model of chronic granulomatous enterocolitis and fibrosis. We used the human colonic CCD-18Co fibroblast/myofibroblast cell line to test the hypothesis that TGF-beta1 and IGF-I interact to regulate proliferation, collagen synthesis, and activated phenotype typified by expression of alpha-smooth muscle actin and organization into stress fibers. IGF-I potently stimulated while TGF-beta1 inhibited basal DNA synthesis. TGF-beta1 and IGF-I each had similar but not additive effects to induce type I collagen. TGF-beta1 but not IGF-I potently stimulated expression of alpha-smooth muscle actin and stress fiber formation. IGF-I in combination with TGF-beta1 attenuated stress fiber formation without reducing alpha-smooth muscle actin expression. Stress fibers were not a prerequisite for increased collagen synthesis. TGF-beta1 upregulated IGF-I mRNA, which led us to examine the effects of IGF-I in cells previously activated by TGF-beta1 pretreatment. IGF-I potently stimulated proliferation of TGF-beta1-activated myofibroblasts without reversing activated fibrogenic phenotype. We conclude that TGF-beta1 and IGF-I both stimulate type I collagen synthesis but have differential effects on activated phenotype and proliferation. We propose that during intestinal inflammation, regulation of activated phenotype and proliferation may require sequential actions of TGF-beta1 and IGF-I, but they may act in concert to increase collagen deposition.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type I,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Peptidoglycan,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/TGFB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0193-1857
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
283
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
G809-18
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12181198-Actins,
pubmed-meshheading:12181198-Animals,
pubmed-meshheading:12181198-Cell Division,
pubmed-meshheading:12181198-Cell Line,
pubmed-meshheading:12181198-Collagen Type I,
pubmed-meshheading:12181198-DNA,
pubmed-meshheading:12181198-Enterocolitis,
pubmed-meshheading:12181198-Fibroblasts,
pubmed-meshheading:12181198-Fibrosis,
pubmed-meshheading:12181198-Humans,
pubmed-meshheading:12181198-Insulin-Like Growth Factor Binding Protein 3,
pubmed-meshheading:12181198-Insulin-Like Growth Factor Binding Proteins,
pubmed-meshheading:12181198-Insulin-Like Growth Factor I,
pubmed-meshheading:12181198-Intestines,
pubmed-meshheading:12181198-Muscle, Smooth,
pubmed-meshheading:12181198-Peptidoglycan,
pubmed-meshheading:12181198-Phenotype,
pubmed-meshheading:12181198-Polysaccharides,
pubmed-meshheading:12181198-Rats,
pubmed-meshheading:12181198-Transforming Growth Factor beta,
pubmed-meshheading:12181198-Transforming Growth Factor beta1
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| pubmed:year |
2002
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| pubmed:articleTitle |
IGF-I and TGF-beta1 have distinct effects on phenotype and proliferation of intestinal fibroblasts.
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| pubmed:affiliation |
Department of Cell and Molecular Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7545, USA. jgs@med.unc.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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