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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-8-7
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pubmed:abstractText |
Much interest has been directed at understanding the adjuvant properties of the heat-labile enterotoxin of Escherichia coli (LT). In this study, we have assessed how LT compared with the nonenzymatic mutant LT (E112K) affect the level of B7-1 and B7-2 expression on APCs, and we determined how these costimulatory molecules influence their adjuvant properties. Analysis of B7-1 and B7-2 expression on B cells revealed that LT enhanced B7-2 but not B7-1, while LT (E112K) had no effect on the expression of either costimulatory molecule. Treatment of macrophage or dendritic cells with LT resulted in a predominant enhancement of B7-2, while LT (E112K) induced mainly B7-1 expression. Analysis of LT- and LT (E112K)-treated B cells, macrophage, and dendritic cells also revealed significant differences in their ability to enhance anti-CD3-stimulated CD4(+) T cell proliferative responses via B7-1 and B7-2. Furthermore, the ability of LT to enhance both Ab and CD4(+) T cell responses to a coadministered Ag was severely abrogated in B7-2- but not B7-1-deficient mice. In contrast, the in vivo adjuvant properties of LT (E112K) appeared to be mediated by both B7-1 and B7-2 for optimal CD4(+) T cell responses, while B7-1 appeared to be the predominant B7 molecule involved in the ability of LT (E112K) to augment Ab responses to a coadministered Ag. These findings demonstrate distinct differences in the ability of LT and LT (E112K) to enhance B7-1 and B7-2 on APC, as well as a dependence upon these costimulatory molecules for their adjuvant properties.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Enterotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alphaXbeta2,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/heat-labile enterotoxin, E coli
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
169
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1744-52
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pubmed:dateRevised |
2008-8-20
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pubmed:meshHeading |
pubmed-meshheading:12165495-Adjuvants, Immunologic,
pubmed-meshheading:12165495-Animals,
pubmed-meshheading:12165495-Antigen-Presenting Cells,
pubmed-meshheading:12165495-Antigens, CD,
pubmed-meshheading:12165495-Antigens, CD45,
pubmed-meshheading:12165495-Antigens, CD80,
pubmed-meshheading:12165495-Antigens, CD86,
pubmed-meshheading:12165495-B-Lymphocytes,
pubmed-meshheading:12165495-Bacterial Toxins,
pubmed-meshheading:12165495-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12165495-Enterotoxins,
pubmed-meshheading:12165495-Escherichia coli,
pubmed-meshheading:12165495-Escherichia coli Proteins,
pubmed-meshheading:12165495-Integrin alphaXbeta2,
pubmed-meshheading:12165495-Lymphocyte Activation,
pubmed-meshheading:12165495-Macrophage-1 Antigen,
pubmed-meshheading:12165495-Membrane Glycoproteins,
pubmed-meshheading:12165495-Mice,
pubmed-meshheading:12165495-Mice, Inbred BALB C,
pubmed-meshheading:12165495-Mice, Knockout,
pubmed-meshheading:12165495-Point Mutation,
pubmed-meshheading:12165495-Up-Regulation
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pubmed:year |
2002
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pubmed:articleTitle |
Role of B7 costimulatory molecules in the adjuvant activity of the heat-labile enterotoxin of Escherichia coli.
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pubmed:affiliation |
Department of Microbiology, University of Alabama, Birmingham, AL 35294, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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