Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-8-6
pubmed:abstractText
ATP-sensitive K+ (K(ATP)) channels couple metabolic changes to membrane excitability in vascular smooth muscle cells (SMCs). While the electrophysiological properties of K(ATP) channels have been examined, little is known about the molecular basis of K(ATP) complex in vascular SMCs. We identified and cloned four K(ATP) subunit genes from rat mesenteric artery, namely rvKir6.1, rvKir6.2, rvKirSUR1, and rvSUR2B. These clones showed over 99.6% amino acid sequence identity with other previously reported isoforms. The mRNA expression patterns of the K(ATP) subunits varied among rat aorta, mesenteric artery, pulmonary artery, tail artery, hepatic artery, and portal vein. Heterologous co-expression of rvKir6.1 and rvSUR2B yielded functional K(ATP) channels that were inhibited by glibenclamide, and opened by pinacidil. Our results for the first time reported the expression of four K(ATP) subunits in same vascular tissues, unmasking the diversity of native K(ATP) channels in vascular SMCs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
296
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
463-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12163042-Adenosine Triphosphate, pubmed-meshheading:12163042-Animals, pubmed-meshheading:12163042-Anti-Arrhythmia Agents, pubmed-meshheading:12163042-Brain, pubmed-meshheading:12163042-Cells, Cultured, pubmed-meshheading:12163042-Cloning, Molecular, pubmed-meshheading:12163042-Glyburide, pubmed-meshheading:12163042-Humans, pubmed-meshheading:12163042-Male, pubmed-meshheading:12163042-Muscle, Smooth, Vascular, pubmed-meshheading:12163042-Patch-Clamp Techniques, pubmed-meshheading:12163042-Pinacidil, pubmed-meshheading:12163042-Potassium Channels, Inwardly Rectifying, pubmed-meshheading:12163042-Protein Subunits, pubmed-meshheading:12163042-Rats, pubmed-meshheading:12163042-Rats, Sprague-Dawley, pubmed-meshheading:12163042-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12163042-Vasodilator Agents
pubmed:year
2002
pubmed:articleTitle
Molecular basis of ATP-sensitive K+ channels in rat vascular smooth muscles.
pubmed:affiliation
Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't