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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2002-7-31
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pubmed:abstractText |
Stromal cell-derived factor-1 (SDF-1) is a chemoattractant involved in hematopoietic progenitor cell (HPC) trafficking to the bone marrow. We studied the role of bone marrow endothelial proteoglycans (PGs) in SDF-1-mediated migration of HPC using a transwell assay. A subclone of progenitor cell line KG-1 (KG-1v) was used, displaying CXCR4-dependent transmigration. Cell surface PGs on bone marrow endothelial cell line 4LHBMEC did not mediate SDF-1-induced transendothelial migration. In contrast, transwell filters precoated with various glycosaminoglycans (GAGs) enhanced migration toward SDF-1. SDF-1-induced migration was reduced by degradation of heparan sulfate in subendothelial matrix produced by 4LHBMEC. The stimulating effect of GAGs was caused by the formation of a stable haptotactic SDF-1 gradient, as SDF-1 bound to immobilized GAGs and triggered migration. Soluble heparan sulfate enhanced SDF-1-induced migration dose-dependently, suggesting that SDF-1-heparan sulfate complexes optimized SDF-1 presentation. In conclusion, we provide evidence that PGs in the subendothelial matrix establish an SDF-1 gradient guiding migrating HPC into the bone marrow.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CXCL12 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Heparitin Sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR4,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0741-5400
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
353-62
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12149427-Bone Marrow,
pubmed-meshheading:12149427-Cell Adhesion,
pubmed-meshheading:12149427-Cell Movement,
pubmed-meshheading:12149427-Cells, Cultured,
pubmed-meshheading:12149427-Chemokine CXCL12,
pubmed-meshheading:12149427-Chemokines, CXC,
pubmed-meshheading:12149427-Dose-Response Relationship, Drug,
pubmed-meshheading:12149427-Endothelium, Vascular,
pubmed-meshheading:12149427-Extracellular Matrix,
pubmed-meshheading:12149427-Fibronectins,
pubmed-meshheading:12149427-HL-60 Cells,
pubmed-meshheading:12149427-Hematopoietic Stem Cells,
pubmed-meshheading:12149427-Heparin,
pubmed-meshheading:12149427-Heparitin Sulfate,
pubmed-meshheading:12149427-Humans,
pubmed-meshheading:12149427-Macromolecular Substances,
pubmed-meshheading:12149427-Osmolar Concentration,
pubmed-meshheading:12149427-Protein Binding,
pubmed-meshheading:12149427-Proteoglycans,
pubmed-meshheading:12149427-Receptors, CXCR4,
pubmed-meshheading:12149427-Solubility,
pubmed-meshheading:12149427-Structure-Activity Relationship,
pubmed-meshheading:12149427-Vascular Cell Adhesion Molecule-1
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pubmed:year |
2002
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pubmed:articleTitle |
Proteoglycans guide SDF-1-induced migration of hematopoietic progenitor cells.
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pubmed:affiliation |
Department of Hematology, Vrije Universiteit Medical Center, Amsterdam, The Netherlands.
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pubmed:publicationType |
Journal Article
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