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rdf:type |
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lifeskim:mentions |
umls-concept:C0003595,
umls-concept:C0008387,
umls-concept:C0023779,
umls-concept:C0023820,
umls-concept:C0074129,
umls-concept:C0243144,
umls-concept:C0332307,
umls-concept:C0456387,
umls-concept:C1145667,
umls-concept:C1413218,
umls-concept:C1880497,
umls-concept:C1996904,
umls-concept:C2349975
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pubmed:issue |
39
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pubmed:dateCreated |
2002-9-23
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pubmed:abstractText |
The Class B type I scavenger receptor I (SR-BI) is a physiologically relevant high density lipoprotein (HDL) receptor that can mediate selective cholesteryl ester (CE) uptake by cells. Direct interaction of apolipoprotein E (apoE) with this receptor has never been demonstrated, and its implication in CE uptake is still controversial. By using a human adrenal cell line (NCI-H295R), we have addressed the role of apoE in binding to SR-BI and in selective CE uptake from lipoproteins to cells. This cell line does not secrete apoE and SR-BI is its major HDL-binding protein. We can now provide evidence that 1) free apoE is a ligand for SR-BI, 2) apoE associated to lipids or in lipoproteins does not modulate binding or CE-selective uptake by the SR-BI pathway, and 3) the direct interaction of free apoE to SR-BI leads to an increase in CE uptake from lipoproteins of both low and high densities. We propose that this direct interaction could modify SR-BI structure in cell membranes and potentiate CE uptake.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD36,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins E,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosides,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, HDL,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lipoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Scavenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SCARB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Scarb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Scavenger Receptors, Class B,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombin,
http://linkedlifedata.com/resource/pubmed/chemical/very high density lipoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/xylosides
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
36092-9
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:12138091-Antigens, CD36,
pubmed-meshheading:12138091-Apolipoproteins E,
pubmed-meshheading:12138091-Binding, Competitive,
pubmed-meshheading:12138091-Binding Sites,
pubmed-meshheading:12138091-Cell Line,
pubmed-meshheading:12138091-Cell Membrane,
pubmed-meshheading:12138091-Cholesterol Esters,
pubmed-meshheading:12138091-Dose-Response Relationship, Drug,
pubmed-meshheading:12138091-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12138091-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12138091-Glycosides,
pubmed-meshheading:12138091-Humans,
pubmed-meshheading:12138091-Hydrolysis,
pubmed-meshheading:12138091-Ligands,
pubmed-meshheading:12138091-Lipid Metabolism,
pubmed-meshheading:12138091-Lipoproteins,
pubmed-meshheading:12138091-Lipoproteins, HDL,
pubmed-meshheading:12138091-Lipoproteins, LDL,
pubmed-meshheading:12138091-Membrane Proteins,
pubmed-meshheading:12138091-Protein Binding,
pubmed-meshheading:12138091-Receptors, Immunologic,
pubmed-meshheading:12138091-Receptors, Lipoprotein,
pubmed-meshheading:12138091-Receptors, Scavenger,
pubmed-meshheading:12138091-Recombinant Proteins,
pubmed-meshheading:12138091-Scavenger Receptors, Class B,
pubmed-meshheading:12138091-Thrombin,
pubmed-meshheading:12138091-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Lipid free apolipoprotein E binds to the class B Type I scavenger receptor I (SR-BI) and enhances cholesteryl ester uptake from lipoproteins.
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pubmed:affiliation |
Unité INSERM U 545, Institut Pasteur de Lille, Faculté de Pharmacie, Université Lille 2, 1 rue du Professeur Calmette, 59019 Lille cedex, France.
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pubmed:publicationType |
Journal Article
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