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rdf:type |
|
|
lifeskim:mentions |
|
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pubmed:issue |
30
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|
pubmed:dateCreated |
2002-7-24
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|
pubmed:abstractText |
We report here the first example using an intein-mediated expression system to generate biotinylated proteins suitable for immobilization onto avidin-functionalized glass slides. With this novel array, proteins are site-specifically immobilized on the glass surface and are able to retain their native activity. The advantage of the avidin/biotin linkage over his-tag/Ni-NTA strategies for protein immobilization is highlighted by its ability to withstand a variety of chemical conditions, which makes this new protein array compatible with most biological assays.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0002-7863
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
124
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pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
8768-9
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
|
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pubmed:year |
2002
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pubmed:articleTitle |
Intein-mediated biotinylation of proteins and its application in a protein microarray.
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|
pubmed:affiliation |
Department of Chemistry and Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore.
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|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
