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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
2002-7-18
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pubmed:abstractText |
The possible modulation of the glutamate transporter EAAC1 by a class A G protein-coupled receptor was studied in transfected C6 glioma cells stably expressing the high-affinity neurotensin receptor NTS1. Brief exposure (5 min) to neurotensin increased Na(+)-dependent D-[(3)H]aspartate uptake by about 70%. The effect of neurotensin was found to result from an increase in cell surface expression of EAAC1 and accordingly, cytochalasin D and colchicine were shown to block the effect of neurotensin on aspartate uptake, suggesting that the cytoskeleton participates in this regulation. Neither protein kinase C nor phosphatidylinositol 3-kinase activities, two intracellular signaling pathways known to modulate EAAC1, was required for EAAC1-mediated aspartate transport regulation by neurotensin. Together, these results provide evidence for an acute regulation of EAAC1 trafficking after activation of a G protein-coupled receptor.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport System X-AG,
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Transporter 3,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Plasma Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Heterotrimeric GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Slc1a1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
523
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
224-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12123836-Amino Acid Transport System X-AG,
pubmed-meshheading:12123836-Animals,
pubmed-meshheading:12123836-Aspartic Acid,
pubmed-meshheading:12123836-Carrier Proteins,
pubmed-meshheading:12123836-Cytoskeleton,
pubmed-meshheading:12123836-Excitatory Amino Acid Transporter 3,
pubmed-meshheading:12123836-GTP-Binding Protein alpha Subunits, Gq-G11,
pubmed-meshheading:12123836-Glutamate Plasma Membrane Transport Proteins,
pubmed-meshheading:12123836-Heterotrimeric GTP-Binding Proteins,
pubmed-meshheading:12123836-Neurotensin,
pubmed-meshheading:12123836-Protein Transport,
pubmed-meshheading:12123836-Rats,
pubmed-meshheading:12123836-Receptors, Cell Surface,
pubmed-meshheading:12123836-Receptors, Neurotensin,
pubmed-meshheading:12123836-Signal Transduction,
pubmed-meshheading:12123836-Symporters,
pubmed-meshheading:12123836-Tumor Cells, Cultured
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pubmed:year |
2002
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pubmed:articleTitle |
Cytoskeleton-related trafficking of the EAAC1 glutamate transporter after activation of the G(q/11)-coupled neurotensin receptor NTS1.
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pubmed:affiliation |
Laboratoire de Pharmacologie Expérimentale, Université Catholique de Louvain, Avenue Hippocrate 54, 1200, Brussels, Belgium. mustapha.najimi@farl.ucl.ac.be
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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