Source:http://linkedlifedata.com/resource/pubmed/id/12111163
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2-3
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pubmed:dateCreated |
2002-7-11
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pubmed:abstractText |
An industrial strain of Saccharomyces cerevisiae (DGI 342) was cultivated in fed-batch cultivations at a specific growth rate of 0.2 h(-1). The yeast was then exposed to carbon or nitrogen starvation for up to 8 h, to study the effect of starvation on fermentative capacity and content of protein, trehalose and glycogen. Nitrogen starvation triggered the accumulation of trehalose and glycogen. After 8 h of starvation, the content of trehalose and glycogen was increased 4-fold and 2-fold, respectively. Carbon starvation resulted in a partial conversion of glycogen into trehalose. The trehalose content increased from 45 to 64 mg (g dry-weight)(-1), whereas the glycogen content in the same period was reduced from 55 to 5 mg (g dry-weight)(-1). Glycogen was consumed faster than trehalose during storage of the starved yeast for 1 month. Nitrogen starvation resulted in a decrease in the protein content of the yeast cells, and the fermentative capacity per gram dry-weight decreased by 40%. The protein content in the carbon-starved yeast increased as a result of starvation due to the fact that the content of glycogen was reduced. The fermentative capacity per gram dry-weight was, however, unaltered.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbon,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycogen,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrogen,
http://linkedlifedata.com/resource/pubmed/chemical/Trehalose
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0175-7598
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
310-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12111163-Biomass,
pubmed-meshheading:12111163-Carbon,
pubmed-meshheading:12111163-Fermentation,
pubmed-meshheading:12111163-Fungal Proteins,
pubmed-meshheading:12111163-Glycogen,
pubmed-meshheading:12111163-Nitrogen,
pubmed-meshheading:12111163-Saccharomyces cerevisiae,
pubmed-meshheading:12111163-Trehalose
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pubmed:year |
2002
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pubmed:articleTitle |
Fed-batch cultivation of baker's yeast followed by nitrogen or carbon starvation: effects on fermentative capacity and content of trehalose and glycogen.
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pubmed:affiliation |
Centre for Process Biotechnology, BioCentrum-DTU, Technical University of Denmark, Building 223, 2800 Kgs. Lyngby, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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