Linked Life Data

12095686

Source:http://linkedlifedata.com/resource/pubmed/id/12095686

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2002-7-3
pubmed:databankReference
pubmed:abstractText
We have determined the complete genomic nucleotide sequence and analyzed the promoter region of murine guanylyl cyclase/natriuretic peptide receptor-A gene (Npr1,coding for NPRA). The gene spans about 17.8 kb and contains 22 exons interrupted by 21 introns. All the exon-intron boundaries possess the consensus GT/AG splice junctions. Four different types of short interspersed nuclear elements (ten mouse B1 elements, seven mouse B2-B4 elements, one ID and one MIR element) and one medium reiteration frequency repeats have been found in the non-coding regions of the gene. Eleven tandem repeats, including three in the promoter region of the gene, have been identified. The transcription start site, 362 bp upstream from the start codon, was determined by 5'- rapid amplification of cDNA ends. The 1.98 kb 5'-flanking region contains three potential SP1 binding sites and one inverted CCAAT box but lacks the TATA box. This region also contains several putative cis-acting motifs known to bind kidney specific nuclear protein HFH-3, cAMP-responsive element binding protein (CREB) and AP-4. In addition, the binding sites for a variety of transcription factors: AML-1 alpha, SRY, Nkx-2.5, LyF-1, p300, GATA-1/2, HNF-3 beta, c/EBP alpha/beta and USF have been localized in the promoter region of Npr1 gene. The analyses and characterization of the genomic structure of murine Npr1 gene should yield important insights into the species-specific regulation of this important gene family.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
291
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
123-33
pubmed:dateRevised
2010-6-25
pubmed:meshHeading
pubmed-meshheading:12095686-5' Flanking Region, pubmed-meshheading:12095686-Animals, pubmed-meshheading:12095686-Base Sequence, pubmed-meshheading:12095686-Blotting, Southern, pubmed-meshheading:12095686-Cloning, Molecular, pubmed-meshheading:12095686-Exons, pubmed-meshheading:12095686-Genes, pubmed-meshheading:12095686-Guanylate Cyclase, pubmed-meshheading:12095686-Introns, pubmed-meshheading:12095686-Mice, pubmed-meshheading:12095686-Mice, Inbred Strains, pubmed-meshheading:12095686-Molecular Sequence Data, pubmed-meshheading:12095686-Promoter Regions, Genetic, pubmed-meshheading:12095686-Receptors, Atrial Natriuretic Factor, pubmed-meshheading:12095686-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:12095686-Sequence Analysis, DNA, pubmed-meshheading:12095686-Sequence Homology, Nucleic Acid, pubmed-meshheading:12095686-Transcription Initiation Site
pubmed:year
2002
pubmed:articleTitle
Genomic structure, organization, and promoter region analysis of murine guanylyl cyclase/atrial natriuretic peptide receptor-A gene.
pubmed:affiliation
Department of Physiology, Tulane University School of Medicine, Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 710112, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.