Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2002-6-27
pubmed:abstractText
Gene expression profiling identified human melanoma cells demonstrating increased cell motility and invasiveness. The gene WNT5A best determined in vitro invasive behavior. Melanoma cells were transfected with vectors constitutively overexpressing Wnt5a. Consistent changes included actin reorganization and increased cell adhesion. No increase in beta-catenin expression or nuclear translocation was observed. There was, however, a dramatic increase in activated PKC. In direct correlation with Wnt5a expression and PKC activation, there was an increase in melanoma cell invasion. Blocking this pathway using antibodies to Frizzled-5, the receptor for Wnt5a, inhibited PKC activity and cellular invasion. Furthermore, Wnt5a expression in human melanoma biopsies directly correlated to increasing tumor grade. These observations support a role for Wnt5a in human melanoma progression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1535-6108
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
279-88
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12086864-Actins, pubmed-meshheading:12086864-Blotting, Western, pubmed-meshheading:12086864-Cell Adhesion, pubmed-meshheading:12086864-Cell Movement, pubmed-meshheading:12086864-Cytoskeletal Proteins, pubmed-meshheading:12086864-Enzyme Inhibitors, pubmed-meshheading:12086864-Gene Expression, pubmed-meshheading:12086864-Gene Expression Regulation, pubmed-meshheading:12086864-Humans, pubmed-meshheading:12086864-Immunoenzyme Techniques, pubmed-meshheading:12086864-Melanoma, pubmed-meshheading:12086864-Neoplasm Invasiveness, pubmed-meshheading:12086864-Polymerase Chain Reaction, pubmed-meshheading:12086864-Precipitin Tests, pubmed-meshheading:12086864-Protein Kinase C, pubmed-meshheading:12086864-Proto-Oncogene Proteins, pubmed-meshheading:12086864-Signal Transduction, pubmed-meshheading:12086864-Skin Neoplasms, pubmed-meshheading:12086864-Trans-Activators, pubmed-meshheading:12086864-Transcription, Genetic, pubmed-meshheading:12086864-Transfection, pubmed-meshheading:12086864-Tumor Cells, Cultured, pubmed-meshheading:12086864-Wnt Proteins, pubmed-meshheading:12086864-beta Catenin
pubmed:year
2002
pubmed:articleTitle
Wnt5a signaling directly affects cell motility and invasion of metastatic melanoma.
pubmed:affiliation
National Human Genome Research Institute, Cancer Genetics Branch, National Institutes of Health, Bethesda, MD 20892, USA.
pubmed:publicationType
Journal Article