Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2002-6-26
pubmed:databankReference
pubmed:abstractText
The events of the cell cycle, the stages at which the cell proliferates and divides, are facilitated and controlled by multiple signaling pathways. Among the many regulatory enzymes that contribute to these processes is the polo-like kinase (Plk). Plks have been reported to mediate multiple mitotic processes, including bipolar spindle formation, activation of Cdc25C, actin ring formation, centrosome maturation, and activation of the anaphase-promoting complex. To investigate its functions in mammalian cells further, we used the recently developed small interfering RNA technique specifically to deplete Plk1 in cultured cells. We find that Plk1 depletion results in elevated Cdc2 protein kinase activity and thus attenuates cell-cycle progression. About 45% of cells treated with Plk1 small interfering RNA show the formation of a dumbbell-like DNA organization, suggesting that sister chromatids are not completely separated. About 15% of these cells do complete anaphase but do not complete cytokinesis. Finally, Plk1 depletion significantly reduces centrosome amplification in hydroxyurea-treated U2OS cells. These data provide direct evidence that Plk is required for multiple mitotic processes in mammalian cells and their significance is discussed.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-10222126, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-10559879, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-10980711, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-11051553, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-11157974, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-11278991, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-11371343, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-11373684, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-11461705, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-11514540, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-11544175, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-11927556, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-11931760, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-1825803, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-3417791, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-7575488, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-7744248, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-8703070, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-8991084, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-9482730, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-9482731, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-9601104, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-9632810, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-9660921, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-9806800, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-9813088, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-9869630, http://linkedlifedata.com/resource/pubmed/commentcorrection/12077309-9933170
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8672-6
pubmed:dateRevised
2011-11-2
pubmed:meshHeading
pubmed-meshheading:12077309-Base Sequence, pubmed-meshheading:12077309-Blotting, Western, pubmed-meshheading:12077309-CDC2 Protein Kinase, pubmed-meshheading:12077309-Cell Cycle, pubmed-meshheading:12077309-Cell Cycle Proteins, pubmed-meshheading:12077309-Centrosome, pubmed-meshheading:12077309-Chromatids, pubmed-meshheading:12077309-Cyclin B, pubmed-meshheading:12077309-DNA Primers, pubmed-meshheading:12077309-HeLa Cells, pubmed-meshheading:12077309-Humans, pubmed-meshheading:12077309-Hydrolysis, pubmed-meshheading:12077309-Molecular Sequence Data, pubmed-meshheading:12077309-Precipitin Tests, pubmed-meshheading:12077309-Protein Kinases, pubmed-meshheading:12077309-Protein-Serine-Threonine Kinases, pubmed-meshheading:12077309-Proto-Oncogene Proteins, pubmed-meshheading:12077309-RNA, Small Interfering, pubmed-meshheading:12077309-RNA, Untranslated
pubmed:year
2002
pubmed:articleTitle
Activation of Cdc2/cyclin B and inhibition of centrosome amplification in cells depleted of Plk1 by siRNA.
pubmed:affiliation
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't