Linked Life Data

12055232

Source:http://linkedlifedata.com/resource/pubmed/id/12055232

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2002-6-10
pubmed:abstractText
Proteins are generally regarded as ineffective immunogens for CTL responses. We synthesized a 100-mer decaepitope polypeptide and tested its capacity to induce multiple CD8(+) IFN-gamma and Th lymphocyte (HTL) responses in HLA transgenic mice. Following a single immunization in the absence of adjuvant, significant IFN-gamma in vitro recall responses were detected for all epitopes included in the construct (six A2.1-, three A11-restricted CTL epitopes, and one universal HTL epitope). Immunization with truncated forms of the decaepitope polypeptide was used to demonstrate that optimal immunogenicity was associated with a size of at least 30-40 residues (3-4 epitopes). Solubility analyses of the truncated constructs were used to identify a correlation between immunogenicity for IFN-gamma responses and the propensity of these constructs to form particulate aggregates. Although the decaepitope polypeptide and a pool of epitopes emulsified in IFA elicited similar levels of CD8(+) responses using fresh splenocytes, we found that the decaepitope polypeptide more effectively primed for in vitro recall CD8(+) T cell responses. Finally, immunogenicity comparisons were also made between the decaepitope polypeptide and a corresponding gene encoding the same polypeptide delivered by naked DNA immunization. Although naked DNA immunization induced somewhat greater direct ex vivo and in vitro recall responses 2 wk after a single immunization, only the polypeptide induced significant in vitro recall responses 6 wk following the priming immunization. These studies support further evaluation of multiepitope polypeptide vaccines for induction of CD8(+) IFN-gamma and HTL responses.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6189-98
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12055232-Animals, pubmed-meshheading:12055232-Buffers, pubmed-meshheading:12055232-CD8-Positive T-Lymphocytes, pubmed-meshheading:12055232-DNA, pubmed-meshheading:12055232-Drug Contamination, pubmed-meshheading:12055232-Emulsions, pubmed-meshheading:12055232-Epitopes, T-Lymphocyte, pubmed-meshheading:12055232-Freund's Adjuvant, pubmed-meshheading:12055232-HLA Antigens, pubmed-meshheading:12055232-Humans, pubmed-meshheading:12055232-Injections, Intramuscular, pubmed-meshheading:12055232-Injections, Subcutaneous, pubmed-meshheading:12055232-Interferon-gamma, pubmed-meshheading:12055232-Jurkat Cells, pubmed-meshheading:12055232-Lymphocyte Activation, pubmed-meshheading:12055232-Mice, pubmed-meshheading:12055232-Mice, Inbred BALB C, pubmed-meshheading:12055232-Mice, Transgenic, pubmed-meshheading:12055232-Peptide Fragments, pubmed-meshheading:12055232-Solubility, pubmed-meshheading:12055232-T-Lymphocytes, Helper-Inducer, pubmed-meshheading:12055232-Transgenes, pubmed-meshheading:12055232-Vaccination, pubmed-meshheading:12055232-Vaccines, Synthetic
pubmed:year
2002
pubmed:articleTitle
A decaepitope polypeptide primes for multiple CD8+ IFN-gamma and Th lymphocyte responses: evaluation of multiepitope polypeptides as a mode for vaccine delivery.
pubmed:affiliation
Epimmune, San Diego, CA 92121, USA. jalexander@epimmune.com
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.