Linked Life Data

12054551

Source:http://linkedlifedata.com/resource/pubmed/id/12054551

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2002-6-10
pubmed:databankReference
pubmed:abstractText
In this study, we report the cloning of porcine UPII genomic DNA, which contains a putative full-length open reading frame encoding the UPII protein. A comparison of the porcine UPII gene coding sequence with the previously published mouse UPII sequence demonstrates that the exon sequences are only partially conserved. Northern and immunohistochemical analyses show that the porcine UPII gene is expressed only in the urothelium and that the protein specifically localizes to urothelial superficial cells. Among urothelial superficial cells, 8.5-9.8% of umbrella cells expresses the UPII gene. A 2-kb region of the porcine UPII promoter contains multiple transcription factor binding sites, including GC-boxes, SP1, AP2, and GATA-box sites, but no TATA or CAAT-box sequences. A sequence comparison of the porcine and murine UPII promoter genes by the MEME system allowed two conserved motifs to be identified, suggesting that these sequences have cis-acting regulatory roles. Sequence homologies between the motifs A and B of the two species are 79% and 80%, respectively, although their relative locations are different. Our results show that the porcine UPII gene is expressed highly and specifically in the bladder urothelium.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2002 Elsevier Science (USA).
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
293
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
862-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Cloning, sequencing, and expression analysis of the porcine uroplakin II gene.
pubmed:affiliation
Division of Applied Life Science, College of Agriculture, Gyeongsang National University, Chinju, GyeongNam 660-701, Republic of Korea [corrected].
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't