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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2002-6-7
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pubmed:abstractText |
B-cell lineage-specific activator protein (BSAP), encoded by the Pax-5 gene, is critical for B-cell lineage commitment and B-cell development but is not expressed in terminally differentiated B cells. We demonstrate a direct connection between BSAP and B-lymphocyte-induced maturation protein 1 (Blimp-1), a transcriptional repressor that is sufficient to drive plasmacytic differentiation. Blimp-1 binds a site on the Pax-5 promoter in vitro and in vivo and represses the Pax-5 promoter in a binding-site-dependent manner. By ectopically expressing Blimp-1 or a competitive inhibitor of Blimp-1, we show that Blimp-1 is both necessary and sufficient to repress Pax-5 during plasmacytic differentiation of primary splenic B cells. Blimp-1-dependent repression of Pax-5 is sufficient to regulate BSAP targets CD19 and J chain and is necessary but not sufficient to induce XBP-1. We further show that repression of Pax-5 is required for Blimp-1 to drive differentiation of splenocytes to immunoglobulin M-secreting cells. Thus, repression of Pax-5 plays a critical role in the Blimp-1-dependent program of plasmacytic differentiation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-10435576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-10524622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-10713181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-10981963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-11067898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-11073970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-11101876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-11120780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-11248811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-11460154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-1375324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-1516825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-1569931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-1730257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-1851123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-2116362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-7511679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-7895521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-8001127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-8021508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-8168136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-8195169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-8497273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-8566078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-8627152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-8885870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-9042861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-9110979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-9120274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-9506950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-9545244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-9649500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-9743352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12052884-9887105
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD19,
http://linkedlifedata.com/resource/pubmed/chemical/B-Cell-Specific Activator Protein,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin J-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Pax5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Prdm1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/regulatory factor X transcription...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4771-80
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12052884-Animals,
pubmed-meshheading:12052884-Antigens, CD19,
pubmed-meshheading:12052884-B-Cell-Specific Activator Protein,
pubmed-meshheading:12052884-B-Lymphocytes,
pubmed-meshheading:12052884-Binding Sites,
pubmed-meshheading:12052884-Cell Differentiation,
pubmed-meshheading:12052884-Cells, Cultured,
pubmed-meshheading:12052884-DNA-Binding Proteins,
pubmed-meshheading:12052884-Gene Expression Regulation,
pubmed-meshheading:12052884-Immunoglobulin J-Chains,
pubmed-meshheading:12052884-Immunoglobulin M,
pubmed-meshheading:12052884-Mice,
pubmed-meshheading:12052884-Promoter Regions, Genetic,
pubmed-meshheading:12052884-Repressor Proteins,
pubmed-meshheading:12052884-Transcription Factors
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pubmed:year |
2002
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pubmed:articleTitle |
Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells.
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pubmed:affiliation |
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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