12036870

Source:http://linkedlifedata.com/resource/pubmed/id/12036870

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0033684, umls-concept:C0086418, umls-concept:C0229601, umls-concept:C0332281, umls-concept:C0870134, umls-concept:C1537064, umls-concept:C1711351
pubmed:issue	12
pubmed:dateCreated	2002-5-30
pubmed:abstractText	The transmembrane 4 superfamily (TM4SF) has come into prominence for its association with a wide range of cell surface molecules, especially integrins. We report that TM4SF molecules CD9, CD63, and CD81 are physically associated with c-kit receptor tyrosine kinase in the human factor-dependent myeloid cell line, MO7e. We characterized this complex using coimmunoprecipitation and colocalization methods. The c-kit coimmunoprecipitated with anti-TM4SF antibodies showed several distinct phenotypes compared to the total c-kit immunoprecipitated with anti-c-kit antibody. These included: (1) higher basal level of tyrosine phosphorylation without elevated kinase activity in the absence of Steel factor (SLF), (2) deficient enhancement of tyrosine phosphorylation and kinase activity in response to SLF, (3) elevated binding rate of SLF shown in chemical cross-linking studies, and (4) little internalization and degradation after SLF treatment. Cocapping studies in living cells showed that c-kit colocalized with TM4SF molecules after SLF stimulation, suggesting confirmation of the biochemical data obtained by the coimmunoprecipitation studies. Colocalization of c-kit with CD81 by SLF was also observed in cord blood CD34(+) cells, suggesting the existence of functional units of c-kit in TM4SF complexes in primary hematopoietic cells. This suggests that some TM4SF members may negatively modulate function of c-kit receptor tyrosine kinase and thus regulate receptor sensitivity to SLF in hematopoietic progenitors.
pubmed:grant	http://linkedlifedata.com/resource/pubmed/grant/R01DK5674, http://linkedlifedata.com/resource/pubmed/grant/R01HL56416, http://linkedlifedata.com/resource/pubmed/grant/R01HL67384
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/7603509
pubmed:citationSubset	AIM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD63, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD81, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD9, http://linkedlifedata.com/resource/pubmed/chemical/CD63 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CD81 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CD9 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Platelet Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit, http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor
pubmed:status	MEDLINE
pubmed:month	Jun
pubmed:issn	0006-4971
pubmed:author	pubmed-author:AkashiMakotoM, pubmed-author:AnzaiNaoyukiN, pubmed-author:BroxmeyerHal EHE, pubmed-author:FukudaSeijiS, pubmed-author:KimYoung-JuneYJ, pubmed-author:LeeYoungheeY, pubmed-author:MantelCharlieC, pubmed-author:YounByung-SBS
pubmed:issnType	Print
pubmed:day	15
pubmed:volume	99
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	4413-21
pubmed:dateRevised	2011-11-17
pubmed:meshHeading	pubmed-meshheading:12036870-Antigens, CD, pubmed-meshheading:12036870-Antigens, CD63, pubmed-meshheading:12036870-Antigens, CD81, pubmed-meshheading:12036870-Antigens, CD9, pubmed-meshheading:12036870-Cell Line, pubmed-meshheading:12036870-Fetal Blood, pubmed-meshheading:12036870-Hematopoietic Stem Cells, pubmed-meshheading:12036870-Humans, pubmed-meshheading:12036870-Membrane Glycoproteins, pubmed-meshheading:12036870-Membrane Proteins, pubmed-meshheading:12036870-Multigene Family, pubmed-meshheading:12036870-Phosphorylation, pubmed-meshheading:12036870-Platelet Membrane Glycoproteins, pubmed-meshheading:12036870-Precipitin Tests, pubmed-meshheading:12036870-Protein Binding, pubmed-meshheading:12036870-Proto-Oncogene Proteins c-kit, pubmed-meshheading:12036870-Stem Cell Factor
pubmed:year	2002
pubmed:articleTitle	C-kit associated with the transmembrane 4 superfamily proteins constitutes a functionally distinct subunit in human hematopoietic progenitors.
pubmed:affiliation	Department of Microbiology/Immunology, Indiana University School of Medicine, Indianapolis, 46202-5254, USA.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, P.H.S.