Linked Life Data

12035932

Source:http://linkedlifedata.com/resource/pubmed/id/12035932

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2002-5-30
pubmed:abstractText
We have identified and cloned a cDNA encoding the first odorant-binding protein isolated from mosquitoes. The protein isolated from female antennae of Culex quinquefasciatus (CquiOBP) was not detected in legs (control tissue) or in antennal extracts from males, and showed mobility in native polyacrylamide gels similar to that of the pheromone-binding protein from Bombyx mori. The open reading frame of the cloned cDNA encoded a hydrophobic signal peptide (24 residues) and an acid mature protein (pI 5.5) of 125 amino acid residues (calculated molecular mass 14,504 Da). The transcript was detected by RT-PCR with antennal, but not with leg tissues. CquiOPB shared the highest amino acid identity with a product deduced from Drosophila melanogaster PBPRP-3 cDNA (58.6%), OBPs from scarab beetles (35%), and moths (28%). In addition, CquiOBP showed the hallmark of insect odorant-binding proteins, the six Cys residues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0098-0331
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
867-71
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Identification and cloning of a female antenna-specific odorant-binding protein in the mosquito Culex quinquefasciatus.
pubmed:affiliation
Honorary Maeda-Duffey Laboratory, University of California Davis, 95616, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't