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rdf:type |
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lifeskim:mentions |
umls-concept:C0014442,
umls-concept:C0017262,
umls-concept:C0026845,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0034987,
umls-concept:C0038323,
umls-concept:C0185117,
umls-concept:C0441472,
umls-concept:C1167622,
umls-concept:C1280500,
umls-concept:C1421315,
umls-concept:C1995013,
umls-concept:C2587213,
umls-concept:C2911684
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pubmed:issue |
6
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pubmed:dateCreated |
2002-5-28
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pubmed:abstractText |
Sterol regulatory element binding protein-1c (SREBP-1c) is a transcription factor that mediates insulin effects on hepatic gene expression. It is itself transcriptionally stimulated by insulin in hepatocytes. Here we show that SREBP-1c mRNA is expressed in adult rat skeletal muscles and that this expression is decreased by diabetes. The regulation of SREBP-1c expression was then assessed in cultures of adult muscle satellite cells. These cells form spontaneously contracting multinucleated myotubes within 7 days of culture. SREBP-1c mRNA is expressed in contracting myotubes. A 4-h treatment with 100 nmol/l insulin increases SREBP-1c expression and nuclear abundance by two- to threefold in myotubes. In cultured myotubes, insulin increases the expression of glycolytic and lipogenic enzyme genes and inhibits the 9-cis retinoic acid-induced UCP3 expression. These effects of insulin are mimicked by adenovirus-mediated expression of a transcriptionally active form of SREBP-1c. We conclude that in skeletal muscles, SREBP-1c expression is sensitive to insulin and can transduce the positive and negative actions of the hormone on specific genes and thus has a pivotal role in long-term muscle insulin sensitivity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Mitochondrial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Srebf1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Sterol Regulatory Element Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/alitretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/mitochondrial uncoupling protein 3
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0012-1797
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1722-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:12031958-Animals,
pubmed-meshheading:12031958-Blotting, Northern,
pubmed-meshheading:12031958-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:12031958-Carrier Proteins,
pubmed-meshheading:12031958-Cells, Cultured,
pubmed-meshheading:12031958-DNA-Binding Proteins,
pubmed-meshheading:12031958-Diabetes Mellitus, Experimental,
pubmed-meshheading:12031958-Gene Expression,
pubmed-meshheading:12031958-Gene Expression Regulation,
pubmed-meshheading:12031958-Glycolysis,
pubmed-meshheading:12031958-Insulin,
pubmed-meshheading:12031958-Ion Channels,
pubmed-meshheading:12031958-Lipids,
pubmed-meshheading:12031958-Liver,
pubmed-meshheading:12031958-Male,
pubmed-meshheading:12031958-Mitochondrial Proteins,
pubmed-meshheading:12031958-Muscle, Skeletal,
pubmed-meshheading:12031958-Muscle Contraction,
pubmed-meshheading:12031958-Protein Isoforms,
pubmed-meshheading:12031958-RNA, Messenger,
pubmed-meshheading:12031958-Rats,
pubmed-meshheading:12031958-Rats, Wistar,
pubmed-meshheading:12031958-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12031958-Sterol Regulatory Element Binding Protein 1,
pubmed-meshheading:12031958-Transcription Factors,
pubmed-meshheading:12031958-Transfection,
pubmed-meshheading:12031958-Tretinoin
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pubmed:year |
2002
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pubmed:articleTitle |
Sterol regulatory element binding protein-1c expression and action in rat muscles: insulin-like effects on the control of glycolytic and lipogenic enzymes and UCP3 gene expression.
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pubmed:affiliation |
UPR 1524 CNRS, Institut Cochin de Génétique Moléculaire, Paris, France. guillet-deniau@cochin.inserm.fr
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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