Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
2002-5-23
pubmed:abstractText
The retinoic acid receptor-related orphan receptor alpha (RORalpha) is critically involved in many physiological functions in several organs. We find that the main RORalpha isoform in the mouse liver is the RORalpha4 isoform, in terms of both mRNA and protein levels, while the RORalpha1 isoform is less abundant. Because hypoxia is a major feature of liver physiology and pathology, we examined the effect of this stress on Rora gene expression and RORalpha transcriptional activity. HepG2 human hepatoma cells were cultured for 24 h under normoxia (20% O2) or hypoxia (10, 2, and 0.1% O2) and the abundance of the Rora transcripts measured by Northern blot and semi-quantitative RT-PCR. Hypoxic HepG2 cells contained more Rora mRNA than controls. This was also observed in rat hepatocytes in primary culture. Cobalt chloride and desferrioxamine also increased the amount of Rora mRNA in HepG2 cells. It is likely that these treatments increase the amount of the RORalpha4 protein in HepG2 cells as evidenced by Western blotting in the case of desferrioxamine. Transient transfection experiments indicated that hypoxia, cobalt chloride, and desferrioxamine all stimulate RORalpha transcriptional activity in HepG2 cells. Hence, we believe that RORalpha participates in the control of gene transcription in hepatic cells and modulates gene expression in response to hypoxic stress.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-10404252, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-10514389, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-10655244, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-10675338, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-10729275, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-10830318, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-10900268, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-11053433, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-11058961, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-11252722, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-11296230, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-11371554, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-11390509, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-11550795, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-11553861, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-11850116, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-2331750, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-2545675, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-3779841, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-466453, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-7537266, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-7739535, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-7823947, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-7891708, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-7916608, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-7926749, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-7935491, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-8065358, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-8137243, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-8308005, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-8344964, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-8521507, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-8602221, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-8660366, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-8702678, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-8810320, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-8858107, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9121434, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9226375, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9278389, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-942051, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9510031, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9520475, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9543393, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9564842, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9748288, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9851961, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9860315, http://linkedlifedata.com/resource/pubmed/commentcorrection/12023888-9862959
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Cobalt, http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers, http://linkedlifedata.com/resource/pubmed/chemical/Deferoxamine, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Receptor Subfamily 1..., http://linkedlifedata.com/resource/pubmed/chemical/ROR1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/RORA protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Receptor Tyrosine Kinase-like..., http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear, http://linkedlifedata.com/resource/pubmed/chemical/Rora protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/cobaltous chloride
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
364
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
449-56
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
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