Source:http://linkedlifedata.com/resource/pubmed/id/12021068
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2002-5-21
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| pubmed:abstractText |
We have shown previously that male germ cell-specific sulfoglycolipid, sulfogalactosylglycerolipid (SGG), is involved in sperm-zona pellucida binding, and that SGG and its desulfating enzyme, arylsulfatase A (AS-A), coexist in the same sperm head area. However, AS-A exists at a markedly low level in sperm as compared to SGG (i.e., 1/400 of SGG molar concentration). In the present study, we investigated whether perturbation of this molar ratio would interfere with sperm-egg interaction. We demonstrated that purified AS-A bound to the mouse sperm surface through its high affinity with SGG. When capacitated, Percoll gradient-centrifuged mouse sperm were treated for 1 h with various concentrations of AS-A, their binding to zona-intact eggs was inhibited in a dose-dependent manner and reached the background level with 63 nM AS-A. This inhibition could be partially explained by an increase in premature acrosome reaction. The acrosome-reacted sperm population of the 63 nM AS-A-treated sperm sample was twice that of the control sample (treated with 63 nM ovalbumin) at 1 h (i.e., 32% vs. 15%) and rose to 53% at 2 h. This induction was presumably due to SGG aggregation attributed to AS-A, existing as a dimer at neutral pH, and could be mimicked by anti-SGG immunoglobulin (Ig) G/IgM + secondary IgG antibody. Drastic inhibition (75%) of in vivo fertilization was also observed in females inseminated with sperm suspension containing 630 nM AS-A as compared to the rate in females inseminated with sperm suspension included with 630 nM ovalbumin. Our results demonstrate a promising potential for AS-A as a nonhormonal, vaginal contraceptive.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cerebroside-Sulfatase,
http://linkedlifedata.com/resource/pubmed/chemical/Contraceptive Agents, Female,
http://linkedlifedata.com/resource/pubmed/chemical/Galactolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/sulfogalactosylglycerolipid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0006-3363
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
66
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1820-7
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12021068-Acrosome Reaction,
pubmed-meshheading:12021068-Animals,
pubmed-meshheading:12021068-Cerebroside-Sulfatase,
pubmed-meshheading:12021068-Contraceptive Agents, Female,
pubmed-meshheading:12021068-Dimerization,
pubmed-meshheading:12021068-Galactolipids,
pubmed-meshheading:12021068-Humans,
pubmed-meshheading:12021068-Hydrogen-Ion Concentration,
pubmed-meshheading:12021068-Immunoglobulin G,
pubmed-meshheading:12021068-Immunoglobulin M,
pubmed-meshheading:12021068-Insemination, Artificial,
pubmed-meshheading:12021068-Liver,
pubmed-meshheading:12021068-Male,
pubmed-meshheading:12021068-Mice,
pubmed-meshheading:12021068-Sperm Capacitation,
pubmed-meshheading:12021068-Sperm-Ovum Interactions,
pubmed-meshheading:12021068-Spermatozoa,
pubmed-meshheading:12021068-Zona Pellucida
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| pubmed:year |
2002
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| pubmed:articleTitle |
Binding of arylsulfatase A to mouse sperm inhibits gamete interaction and induces the acrosome reaction.
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| pubmed:affiliation |
Hormones/Growth/Development Group, Ottawa Health Research Institute, Ottawa, Ontario, Canada K1Y 4E9.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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