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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-5-20
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pubmed:abstractText |
Fusions between the Drosophila hsp70 promoter and three different incomplete P elements, KP, SP, and BP1, were inserted into the Drosophila genome by means of hobo transformation vectors and the resulting transgenic stocks were tested for repression of P-element transposase activity. Only the H(hsp/KP) transgenes repressed transposase activity, and the degree of repression was comparable to that of a naturally occurring KP element. The KP transgenes repressed transposase activity both with and without heat-shock treatments. Both the KP element and H(hsp/KP) transgenes repressed the transposase activity encoded by the modified P element in the P(ry(+), Delta2-3)99B transgene more effectively than that encoded by the complete P element in the H(hsp/CP)2 transgene even though the P(ry(+), Delta2-3)99B transgene was the stronger transposase source. Repression of both transposase sources appeared to be due to a zygotic effect of the KP element or transgene. There was no evidence for repression by a strictly maternal effect; nor was there any evidence for enhancement of KP repression by the joint maternal transmission of H(hsp/KP) and H(hsp/CP) transgenes. These results are consistent with the idea that KP-mediated repression of P-element activity involves a KP-repressor polypeptide that is not maternally transmitted and that KP-mediated repression is not strengthened by the 66-kD repressor produced by complete P elements through alternate splicing of their RNA.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-115009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-12019234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-1333435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-1651170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-17248751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-17249100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-1852009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-1965103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-2164887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-2540958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-2558959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-2832152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-2835286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-2840331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-2852140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-3000622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-3030879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-3100389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-6295641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-6309410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-8157003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-8224815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-8224830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-8384145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-8647395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-8816474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-8978042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12019235-9670031
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
205-15
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:12019235-Animals,
pubmed-meshheading:12019235-Animals, Genetically Modified,
pubmed-meshheading:12019235-Crosses, Genetic,
pubmed-meshheading:12019235-DNA Transposable Elements,
pubmed-meshheading:12019235-Drosophila melanogaster,
pubmed-meshheading:12019235-Female,
pubmed-meshheading:12019235-Gene Expression Regulation,
pubmed-meshheading:12019235-Male,
pubmed-meshheading:12019235-Mutation,
pubmed-meshheading:12019235-Transgenes,
pubmed-meshheading:12019235-Transposases
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pubmed:year |
2002
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pubmed:articleTitle |
Regulation of P-element transposase activity in Drosophila melanogaster by hobo transgenes that contain KP elements.
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pubmed:affiliation |
Department of Genetics, Cell Biology and Development, University of Minnesota, Saint Paul, Minnesota 55108-1095, USA. simmo004@tc.umn.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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