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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5574
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pubmed:dateCreated |
2002-6-7
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pubmed:abstractText |
Lymphocyte motility is vital for trafficking within lymphoid organs and for initiating contact with antigen-presenting cells. Visualization of these processes has previously been limited to in vitro systems. We describe the use of two-photon laser microscopy to image the dynamic behavior of individual living lymphocytes deep within intact lymph nodes. In their native environment, T cells achieved peak velocities of more than 25 micrometers per minute, displaying a motility coefficient that is five to six times that of B cells. Antigenic challenge changed T cell trajectories from random walks to "swarms" and stable clusters. Real-time two-photon imaging reveals lymphocyte behaviors that are fundamental to the initiation of the immune response.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
7
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pubmed:volume |
296
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1869-73
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12016203-Adoptive Transfer,
pubmed-meshheading:12016203-Animals,
pubmed-meshheading:12016203-Antigen-Presenting Cells,
pubmed-meshheading:12016203-Antigens,
pubmed-meshheading:12016203-B-Lymphocytes,
pubmed-meshheading:12016203-Cell Division,
pubmed-meshheading:12016203-Cell Movement,
pubmed-meshheading:12016203-Cell Size,
pubmed-meshheading:12016203-Fluoresceins,
pubmed-meshheading:12016203-Fluorescent Dyes,
pubmed-meshheading:12016203-Image Processing, Computer-Assisted,
pubmed-meshheading:12016203-Lasers,
pubmed-meshheading:12016203-Lymph Nodes,
pubmed-meshheading:12016203-Lymphocyte Activation,
pubmed-meshheading:12016203-Mice,
pubmed-meshheading:12016203-Mice, Inbred BALB C,
pubmed-meshheading:12016203-Microscopy,
pubmed-meshheading:12016203-Motion Pictures as Topic,
pubmed-meshheading:12016203-Photons,
pubmed-meshheading:12016203-Rhodamines,
pubmed-meshheading:12016203-Succinimides,
pubmed-meshheading:12016203-T-Lymphocytes,
pubmed-meshheading:12016203-Temperature
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pubmed:year |
2002
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pubmed:articleTitle |
Two-photon imaging of lymphocyte motility and antigen response in intact lymph node.
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pubmed:affiliation |
Department of Physiology and Biophysics, University of California, Irvine, CA 92697-4561, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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