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rdf:type |
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lifeskim:mentions |
umls-concept:C0001128,
umls-concept:C0001962,
umls-concept:C0015127,
umls-concept:C0017262,
umls-concept:C0017725,
umls-concept:C0024188,
umls-concept:C0033808,
umls-concept:C0038382,
umls-concept:C0242726,
umls-concept:C0301869,
umls-concept:C0596311,
umls-concept:C0765250,
umls-concept:C0911550,
umls-concept:C1171362,
umls-concept:C1314792,
umls-concept:C1330957,
umls-concept:C1515670,
umls-concept:C1522492
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pubmed:issue |
1
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pubmed:dateCreated |
2002-5-15
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pubmed:abstractText |
4-Hydroxycinnamoyl-CoA hydratase/lyase (HCHL), a crotonase homologue of phenylpropanoid catabolism from Pseudomonas fluorescens strain AN103, led to the formation of 4-hydroxybenzaldehyde metabolites when expressed in hairy root cultures of Datura stramonium L. established by transformation with Agrobacterium rhizogenes. The principal new compounds observed were the glucoside and glucose ester of 4-hydroxybenzoic acid, together with 4-hydroxybenzyl alcohol- O-beta- D-glucoside. In lines actively expressing HCHL, these together amounted to around 0.5% of tissue fresh mass. No protocatechuic derivatives were found, although a trace of vanillic acid-beta- D-glucoside was detected. There was no accumulation of 4-hydroxybenzaldehydes, whether free or in the form of their glucose conjugates. There was some evidence suggesting a diminished availability of feruloyl-CoA for the production of feruloyl putrescine and coniferyl alcohol. The findings are discussed in the context of a diversion of phenylpropanoid metabolism, and the ability of plants and plant cultures to conjugate phenolic compounds.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-phenylpropanol,
http://linkedlifedata.com/resource/pubmed/chemical/4-hydroxybenzaldehyde,
http://linkedlifedata.com/resource/pubmed/chemical/4-hydroxybenzoic acid,
http://linkedlifedata.com/resource/pubmed/chemical/4-hydroxycinnamoyl-CoA...,
http://linkedlifedata.com/resource/pubmed/chemical/Benzaldehydes,
http://linkedlifedata.com/resource/pubmed/chemical/Enoyl-CoA Hydratase,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Hydro-Lyases,
http://linkedlifedata.com/resource/pubmed/chemical/Lignin,
http://linkedlifedata.com/resource/pubmed/chemical/Parabens,
http://linkedlifedata.com/resource/pubmed/chemical/Phenols,
http://linkedlifedata.com/resource/pubmed/chemical/Propanols,
http://linkedlifedata.com/resource/pubmed/chemical/Vanillic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/coniferyl alcohol
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0032-0935
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
215
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-89
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12012244-Benzaldehydes,
pubmed-meshheading:12012244-Datura stramonium,
pubmed-meshheading:12012244-Enoyl-CoA Hydratase,
pubmed-meshheading:12012244-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:12012244-Glucose,
pubmed-meshheading:12012244-Hydro-Lyases,
pubmed-meshheading:12012244-Lignin,
pubmed-meshheading:12012244-Parabens,
pubmed-meshheading:12012244-Phenols,
pubmed-meshheading:12012244-Plant Roots,
pubmed-meshheading:12012244-Plants, Genetically Modified,
pubmed-meshheading:12012244-Propanols,
pubmed-meshheading:12012244-Pseudomonas fluorescens,
pubmed-meshheading:12012244-Vanillic Acid
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pubmed:year |
2002
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pubmed:articleTitle |
4-Hydroxycinnamoyl-CoA hydratase/lyase, an enzyme of phenylpropanoid cleavage from Pseudomonas, causes formation of C(6)-C(1) acid and alcohol glucose conjugates when expressed in hairy roots of Datura stramonium L.
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pubmed:affiliation |
Food Safety Science Division, Institute of Food Research, Norwich Resesrch Park, Colney, Norwich NR4 7UA, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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